Human Apolipoprotein CII ELISA Kit（载脂蛋白Apo-CII酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：0.1 μg/ml

检测方法 ：ELISA

应用 ：血清、血浆、细胞培养上清液

样本 ：50 μl

规格 ：96 wells

Introduction
Apolipoprotein C-II (ApoC-II) is secreted in plasma and resides in both the very low density lipoproteins (VLDL) and high density lipoproteins (HDL). ApoC-II plays a major role in lipid metabolism as the obligate cofactor for lipoprotein lipase, which catalyzes the hydrolysis of triglyceride-rich lipoproteins (1). The protein has 79 amino acid residues and, in the absence of lipid, self-associates to form amyloid fibrils implicated in the pathogenesis of a number of diseases including Alzheimer's, Parkinson's, and Creutzfeldt-Jakob diseases (2). ApoC-II aggregates are present in human atherosclerotic plaques, and its fibrils initiate macrophage inflammatory responses (3). Deficiency of either apoC-II or lipoprotein lipase results in hypertriglyceridemia (4). Transgenic mice over-expression of human apoC-II also causes hypertriglyceridemia attributed to the delayed clearance of VLDL triglycerides (5).

Principle of the Assay
The AssayMax Human Apolipoprotein C-II ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human apoC-II in plasma, serum, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human apoC-II in less than 4 hours. A polyclonal antibody specific for human apoC-II has been pre-coated onto a 96-well microplate with removable strips. ApoC-II in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for apoC-II, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.