Human Apolipoprotein AII ELISA Kit（高敏载脂蛋白Apo-AII酶联免疫试剂盒）

库存 ：大量

供应商 ：AssayPro

检测限 ：1.8 ng/ml

检测方法 ：ELISA

应用 ：血清、血浆、细胞培养上清液、细胞裂解液

样本 ：50 μl

规格 ：96 wells

Introduction
Apolipoprotein A-II (ApoA-II) is the second most abundant apolipoprotein in human plasma HDL, comprising about 25% of the protein mass. After being synthesized by the liver and intestine as a preprotein containing 100 amino acids, apoA-II is processed to 77 amino acids in the mature plasma protein (1 - 3). ApoA-II is found in plasma as a monomer, homodimer of 17.4 kDa, or heterodimer with ApoE and ApoD (4 - 7). It has been reported that apoA-II plays roles in HDL remodeling, cholesterol efflux, modulating HDL interaction with enzymes and receptors, triglyceride metabolism, and atherosclerosis (7 - 12). ApoA-II is inversely associated with risk of future coronary artery disease (13). Serum levels of an isoform of apoA-II have been identified as a potential marker for prostate cancer (14).

Principle of the Assay
The AssayMax Human Apolipoprotein A-II ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human apoA-II in plasma, serum, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human apoA-II in less than 4 hours. A polyclonal antibody specific for human apoA-II has been pre-coated onto a 96-well microplate with removable strips. ApoA-II in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for human apoA-II, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.