磷酸二酯酶5A(PDE5A) 检测试剂盒

Kit Summary:
• Detection method- Fluorescence (Ex/Em 540/580 nm)
• Sample type- Cell and Tissue culture lysates.
• Species reactivity- Mammalian
• Applications- A sensitive and high speed homogeneous assay for detection of enzyme activity and for screening of Phosphodiesterase 5A (PDE5A) inhibitors in a high-throughput screening (HTS) format.

Features & Benefits:
• Simple procedure
• Fast and convenient

Kit components:
• Assay Buffer
• Sensor Stock
• Sensor Dilution Buffer
• cGMP Substrate
• GMP Calibrator
• 384 well plate

Description:
The PhosphoSeek™ Technology for screening of phosphodiesterase activities is a robust and homogeneous detection platform that measures the activity of a target enzyme. Assays are non-competitive with respect to substrate and do not require radioactive materials or secondary (detector) enzymes or antibodies. Assays can be performed using defined reaction components (biochemical assay) or for detection of endogenous enzyme activities using cellular lysates. Enzyme progress can be monitored after reactions have occurred or in continuous mode as substrate conversion occurs in real time. The assays are ideally suited for automated screening and can be read on any fluorometer. The Sensor is a proprietary molecule that contains a trivalent metal ion, which binds to phosphoryl groups on biological substrates. Activity is measured by the change of fluorescence of a dye-labeled cyclic guanosine monophosphate (cGMP) upon hydrolysis and binding by Sensor. The change in fluorescence directly correlates to the level of substrate conversion.

Storage Conditions:
MT

Shipping Conditions:

USAGE: For Research Use Only! Not For Use in Humans.