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Promega 细胞健康检测方案
想您所想，为您提供更多!
Promega作为细胞健康检测方案的可靠供应商，产品覆盖了细胞活力检测、毒性检测、细胞凋亡检测、谷胱甘肽测定等多个方面。
针对同一种细胞健康指标，我们提供多种方法，包括比色法、荧光法、生物发光法的检测方法，以满足您的不同需求。
特点
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Promega拥有的生物发光技术，可提供更灵敏，更快速的解决方案
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操作可简单至“加样-混合-检测”一步法，最快5-10分钟完成
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拥有多重检测技术，为单一细胞孔，提供更多检测指标数据
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丰富的产品线，总有一款适合您已有的检测设备
microscopy
| 检测类型 | 检测方法 | 目录号 | 检测参数/ 生物标记 |
检测所 需时间 |
灵敏度 | 平板模式 | 仪器 | ||
| 细胞活力 |
|
G7570/G7571/ G7572/G7573 |
ATP | 10 min | 10 living cells | 96/384/1536 | Luminometer/CCD | ||
|
G8461/G8462 | ATP | 10 min | 10 living cells | 96/384/1536 | Luminometer/CCD | |||
|
G6080/G6081/ G6082 |
Live-cell protease | 0.5–3 h | 40 living cells | 96/384/1536 | Fluorometer,AFC 400Ex/505Em |
|||
|
G8080/G8081/ G8082 |
Resazurin reduction by reducing equivalents | 1–4 h | 400 living cells | 96/384/1536 | Fluorometer,resorufin 560Ex/590Em |
|||
|
G3580/G3581/ G3582 |
MTS reduction by reducing equivalents | 1–4 h | 1,000 living cells | 96/384 | Spectrophotometer Abs 490 nm |
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|
G4000/G4100 | MTT reduction by reducing equivalents | 5h | >1,000 living cells | 96/384 | Spectrophotometer Abs 570 nm |
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|
G8230/G8231/ G8232/G8233 |
ATP | 5 min | 10 living bacteria | 96/384 | Luminometer | |||
| 细胞凋亡 |
|
G0940/G0941 | Caspase-2 activity | 0.5 h | Enzyme preparation | 96/384/1536 | Luminometer | ||
|
G8200/G8201/ G8202 |
Caspase-8 activity | 0.5 h | ~1,000 apoptotic cells | 96 | Luminometer | |||
|
G8210/G8211/ G8212 |
Caspase-9 activity | 0.5 h | ~1,500 apoptotic cells | 96 | Luminometer | |||
|
G7790/G7791/ G7792 |
Caspase-3/7 activity | 1–18 h | 625 apoptotic cells | 96/384/1536 | Fluorometer,R110, 499Ex/521Em |
|||
|
G8090/8091/ G8092/G8093 |
Caspase-3/7 activity | 0.5 h | 100 apoptotic cells | 96/384/1536 | Luminometer | |||
|
G0970/G0971 | Caspase-6 activity | 0.5 h | Enzyme preparation | 96/384/1536 | Luminometer | |||
|
G7351/G7220 | caspase-3 activity | 5h | Enzyme preparation, cell extracts,tissue lysates | 96 | Spectrophotometer Abs 405nm |
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|
G7461/G7462 | caspase activity | 1.5h | In situ detection | 96 | florescence microscopy, flow cytometry |
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|
G7360/G7130 | nuclear-DNA fragmentation | In situ detection | ||||||
|
G3250 | nuclear-DNA fragmentation | In situ detection at single cell level or cell suspension | florescence microscopy, flow cytometry |
|||||
| 细胞毒性 |
|
G9290/G9291/ G9292 |
Dead-cell protease release | 15 min | 10 dead cells | 96/384/1536 | Luminometer | ||
|
G9260/G9261/ G9262 |
Dead-cell protease release | 0.5–3 h | 10 dead cells | 96/384 | Fluorometer,R110 485Ex/520Em |
|||
|
G7890/G7891/ G7892 |
LDH release | 10 min | 200 dead cells | 96/384 | Fluorometer,resorufin 560Ex/590Em | |||
|
G1780 | LDH release | 30-60min | 96 | Spectrophotometer Abs 490 nm |
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|
G8741/G8742/ G8743/G8731 |
DNA binding by cell impermeable dye | 15min | 50 dead cells | 96/384/1536 | Fluorometer, 485Ex/520Em Proprietary dye |
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| 多重检测 |
|
G9270/G9271/ G9272 |
Viability + cytotoxicity;live- + dead-cell protease | 0.5 h | 40 living cells,10 dead cells | 96/384/1536 | Fluorometer,AFC 400Ex/505Em Luminometer |
||
|
G9200/G9201/ G9202 |
Viability + cytotoxicity;live- + dead-cell protease | 0.5–3 h | 40 living cells,10 dead cells | 96/384/1536 | Fluorometer,AFC 400Ex/505Em R110 485Ex/520Em |
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|
G6410/G6411 | Viability + apoptosis;live-cell protease+ caspase-3/7 | 1–3 h | ~40 living cells,100 apoptotic cells | 96/384 | Fluorometer,AFC 400Ex/505Em Luminometer |
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|
G6320/G6321 | Viability, cytotoxicity + apoptosis live- + dead-cell protease +caspase-3/7 | 1–3 h | ~40 living cells,100 apoptotic cells | 96/384 | Fluorometer,AFC 400Ex/505Em R110 485Ex/520Em Luminometer |
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|
E7110/E7120 | Viability + reporter geneexpression;live-cell protease + luciferase activity | 0.6–3 h | ~40 living cells | 96/384 | Fluorometer,AFC 400Ex/505Em Luminometer |
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| 线粒体功能 |
|
G8000/G8001 | Mitochondrial toxicity; deadcell protease + ATP;(Crabtree effect, galactose medium) | 0.6–3 h | 96/384 | Fluorometer,R110 485Ex/520Em Luminometer |
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| 谷胱甘肽测定 |
|
V6911/V6912 | GSH | 30 minutes | 96/384 | Luminometer | |||
|
V6611/V6612 | GSH/GSSG | 1 hour | 96/384 | Luminometer |
厦门泰京生物技术有限公司
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