QuantiCyto® Human IFN-γ ELISA kit（人γ干扰素）价格

库存：大量

供应商：欣博盛生物科技有限公司

检测限：15.6-1000pg/ml

检测方法：双抗体检测方法

应用：ELISA试剂盒

适应物种：人

标记物：HRP标记

样本：血清/ 血浆/ 细胞培养上清

规格：96T

高品质国产ELISA试剂盒——欣博盛QuantiCyto^® ELISA试剂盒

欣博盛生物科技有限公司是一家致力于生命科学和生物技术领域的高科技企业。自2007年成立以来，专注于为科研工作者提供高品质的细胞生物学、分子生物学和免疫学相关产品以及专业的服务。公司由在国内科研试剂领域有着十几年从业经验的专业技术团队和企业管理团队组建而成，欣博盛自主研发的ELISA试剂盒，品质卓越，行业领先；在国内众多重点实验室广泛使用，深受广大科研人员的好评。欣博盛品牌 ELISA试剂盒自推出以来已先后在Nature, Advanced materials, PNAS, Nature Immunology, Cell research等国际权威杂志文章中被引用。

人IFN-γ简介：
人IFN-γ为21-24KDa的亚基构成的同二聚体，与IFN-α,β不同，IFN-γ的合成只能在T细胞和NK细胞。它可增加单核细胞的吞噬和吞饮作用，刺激细胞通过释放氧化因子和TNF-a 而杀灭肿瘤细胞，它可增加激活B细胞的繁殖，与IL-2协同增加免疫球蛋白轻链的合成。此外IFN-γ激活中性粒细胞，NK细胞，血管内皮细胞，在许多病理情况下，IFN-γ作为疾病的标志物的作用已得到证实。病毒感染时，IFN-γ产生。IFN-γ可作为鉴别结核性与非结核性腹水的诊断工具。IFN-γ在结核性腹水中的浓度显著高于非结核性腹水,灵敏度和特异性均达到100％。IFN-γ对多发性硬化症的免疫治疗的设计及检测有重要意义。在移植物排斥反应临床症状出现前，IFN-γ的表达量增加，I型糖尿病的初期，IFN-γ的产生显著下降。

检测原理:
欣博盛 QuantiCyto^® ELISA试剂盒采用双抗体夹心法：抗人IFN-γ单抗包被于酶标板上，标本和标准品中的人IFN-γ会与单抗结合，游离的成分被洗去。加入生物素化的抗人IFN-γ抗体和辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合；抗人IFN-γ抗体与结合在单抗上的人IFN-γ结合而形成免疫复合物，游离的成分被洗去。加入显色底物，若反应孔中有人IFN-γ，辣根过氧化物酶会使无色的显色剂现蓝色，加终止液变黄。在450 nm处测OD值，人IFN-γ浓度与OD₄₅₀值之间呈正比，可通过绘制标准曲线求出标本中人IFN-γ的浓度。

为什么选择欣博盛QuantiCyto^® ELISA Kit？

权威认可
1200余篇SCI文献引用，四次登上顶-级期刊Nature！

简便快捷
450nm酶标仪检测，包含ELISA所需全部试剂和详细操作说明，全部现货！

品种齐全
提供200多种常用试剂盒，能检测人、大鼠、小鼠、猴、猪、犬和牛等多种属。

使用广泛
客户遍及全国各地，包括中科院，北京生命科学研究院，北京大学，清华大学，复旦大学，上海交通大学，华中科技大学，浙江大学，南开大学，北京师范大学，第四军医大学，厦门大学，中山大学，南方医科大学，深圳大学，四川大学华西医院，澳门大学，哈尔滨医科大学等著名高校及研究机构。恒瑞医药、海正药业等药物研发企业，荷兰阿姆斯特丹大学等海外研究机构。

完整试剂盒于4°C保存，标准品无需保存于-20°C，方便运输和保存
液体组分提供富余20%体积，足够多次预实验与正式实验使用

注：QuantiCyto^®为欣博盛ELISA试剂盒注册商标

四篇Nature文献引用

Zhou P, She Y, Dong N, et al. Alpha-kinase 1 is a cytosolic innate immune receptor for bacterial ADP-heptose. Nature(2018)
Shi J, Zhao Y, Wang K, et al. Cleavage of gsdmd by inflammatory caspases determines pyroptotic cell death. Nature(2015)
Xu H, Yang J, Gao W, et al.Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome. Nature(2014)
Zhao Y, Yang J, Shi J, et al. The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature(2011)

Human IFN-γ ELISA kit 文献引用：
1.Hu, Fanlei, et al. "Hypoxia and hypoxia-inducible factor-1α provoke toll-like receptor signalling-induced inflammation in rheumatoid arthritis." Annals of the rheumatic diseases 73.5 (2014): 928-936.
2.Zhao, Yu, et al. "Treatment with humanized selective CD19CAR-T cells shows efficacy in highly treated B-ALL patients who have relapsed after receiving murine-based CD19CAR-T therapies." Clinical Cancer Research 25.18 (2019): 5595-5607.
3.Wu, Dan, et al. "NK Cell-Encapsulated Porous Microspheres via Microfluidic Electrospray for Tumor Immunotherapy." ACS applied materials & interfaces (2019).
4.Zhang, Donglei, et al. "The defective bone marrow-derived mesenchymal stem cells in patients with chronic immune thrombocytopenia." Autoimmunity 47.8 (2014): 519-529.
5.Zhou, Xikun, et al. "Cellular immunotherapy for carcinoma using genetically modified EGFR-specific T lymphocytes." Neoplasia (New York, NY) 15.5 (2013): 544.
6.Pian, Yaya, et al. "Phenol-soluble modulin α4 mediates Staphylococcus aureus-associated vascular leakage by stimulating heparin-binding protein release from neutrophils." Scientific reports 6 (2016): 29373.
7.Feng, Min, et al. "The preferential infection of astrocytes by enterovirus 71 plays a key role in the viral neurogenic pathogenesis." Frontiers in cellular and infection microbiology6 (2016): 192.
8.Yang, Yang, et al. "5-Aminolevulinic Acid-Mediated Sonodynamic Therapy Alleviates Atherosclerosis via Enhancing Efferocytosis and Facilitating a Shift in the Th1/Th2 Balance Toward Th2 Polarization." Cellular Physiology and Biochemistry 47.1 (2018): 83-96.
9.Li, Huiyuan, et al. "Numerical and functional defects in CD8+ CD28− T‐suppressor lymphocytes from patients with primary immune thrombocytopenia." British journal of haematology178.2 (2017): 292-301.
10.Fan, Xing-Liang, et al. "Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients." Stem cell research & therapy 9.1 (2018): 170.

该产品被引用文献

1.Hu, Fanlei, et al. "Hypoxia and hypoxia-inducible factor-1α provoke toll-like receptor signalling-induced inflammation in rheumatoid arthritis." Annals of the rheumatic diseases 73.5 (2014): 928-936.
2.Zhao, Yu, et al. "Treatment with humanized selective CD19CAR-T cells shows efficacy in highly treated B-ALL patients who have relapsed after receiving murine-based CD19CAR-T therapies." Clinical Cancer Research 25.18 (2019): 5595-5607.
3.Wu, Dan, et al. "NK Cell-Encapsulated Porous Microspheres via Microfluidic Electrospray for Tumor Immunotherapy." ACS applied materials & interfaces (2019).
4.Zhang, Donglei, et al. "The defective bone marrow-derived mesenchymal stem cells in patients with chronic immune thrombocytopenia." Autoimmunity 47.8 (2014): 519-529.
5.Zhou, Xikun, et al. "Cellular immunotherapy for carcinoma using genetically modified EGFR-specific T lymphocytes." Neoplasia (New York, NY) 15.5 (2013): 544.
6.Pian, Yaya, et al. "Phenol-soluble modulin α4 mediates Staphylococcus aureus-associated vascular leakage by stimulating heparin-binding protein release from neutrophils." Scientific reports 6 (2016): 29373.
7.Feng, Min, et al. "The preferential infection of astrocytes by enterovirus 71 plays a key role in the viral neurogenic pathogenesis." Frontiers in cellular and infection microbiology6 (2016): 192.
8.Yang, Yang, et al. "5-Aminolevulinic Acid-Mediated Sonodynamic Therapy Alleviates Atherosclerosis via Enhancing Efferocytosis and Facilitating a Shift in the Th1/Th2 Balance Toward Th2 Polarization." Cellular Physiology and Biochemistry 47.1 (2018): 83-96.
9.Li, Huiyuan, et al. "Numerical and functional defects in CD8+ CD28− T‐suppressor lymphocytes from patients with primary immune thrombocytopenia." British journal of haematology178.2 (2017): 292-301.
10.Fan, Xing-Liang, et al. "Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients." Stem cell research & therapy 9.1 (2018): 170.