去内毒素质粒小量提取试剂盒

库存 ：大量

英文名 ：Plasmid Mini Kit

供应商 ：研卉生物

规格 ：100

去内毒素质粒小量提取试剂盒
小量提取时，本试剂盒一次可以提取1-5ml菌液。根据质粒、菌种的不同而表现出不同的质粒提取量，一般提取量在1μg--8μg/ml菌液。中量提取时，一个离心柱可从15ml菌液中，最多提取到80μg质粒DNA。该产品采用本公司特有的DNA-only硅胶膜离心柱和配方，不需添加RNA酶，即可除去RNA，使实验室免受RNA酶的污染。内毒素﹤8U/μgDNA。


产品内容

DNA-only硅胶膜离心柱、BufferS1、BufferS2、Buffer S3、Buffer PW、Buffer WB、Buffer EB。


产品特点

型号	离心柱型	纯化组件	Foregene硅胶膜离心柱、试剂
通量	1-24个样品	制备时间	20min（24个样品）
仪器需求	台式离心机	细菌裂解物分离	离心分离
离心柱液体盛装量	800μl	DNA-only硅胶膜离心柱DNA承载量	80μg
最小洗脱体积	50μl	菌液处理量	1-15ml

产品应用
Plasmid Mini Kit用于从细菌中快速分离提取高质量的质粒DNA；获得的质粒DNA可用于下游分子生物学实验，如：酶切、PCR、文库构建、测序、转化、传代细胞甚至原代细胞转染等。


本试剂盒提取获得的质粒DNA，其OD260/280稳定在1.7—1.9之间。

用户反馈

用户来自：西安交通大学医学院第一附属医院心内科 吕颖

使用产品：小提质粒试剂盒

使用评价：该试剂盒操作简易方便，操作说明书使用步骤简单易懂，不需额外加用试剂，该试剂盒所配置的耗材可以和实验室常规的耗材很方便的衔接使用，且操作中能和离心机配套使用且不用对离心机更换大小转子及转速做很多调试，操作中带给使用者更多的方便，省时省力，得心应手。提取的高纯度和高浓度的质粒，经过跑胶超螺旋影像清晰可见，并将GFP质粒进行转染后绿色荧光满视野，效率很高，对细胞的损伤也很小，比同类试剂盒在操作流程及质粒使用中显示的效果方面，有很大的优势和前景，当然也存在一些小瑕疵，该试剂盒可以在去除内毒素和RNA方面做点工作，将产品质量再提高到新的高度，相信贵公司经更优化的开发会有更高品质的质粒提取试剂盒应运而生，并开拓更大的市场！世上没有十全十美，希望贵公司更加努力生产出更多更优质的产品！没有最好只有更好！科研无极限！不断突破！

User From：Postdoctoral Associate in Garen Laboratory LingLi
Department of Molecular Biophysics and Biochemistry, Yale Medicine School
Product：Plasmid Mini Kit
Evaluation：Following the procedures provided by the user handbook, we evaluated the application of the Plasmid Mini Kit, produced by Foregene Biotechnology Co., Ltd., on small-scale plasmid DNA purification, and the especially introduced feature of removing the RNA component involved in the E.coli lysate without the addition of RNase. After the test using E.coli JM109 transformed with plasmid PMD19-T-A as material (A means an inserted gene), we provide the following viewpoints as our objective evaluation.

1. This Plasmid Mini Kit provides a fast, simple, and convenient plasmid processing of 1-20 samples simultaneously in less than 25minutes.

2. The RNA component involved in the lysate sample can be removed completely without the addition of RNase (As shown by electrophoretogram). And the closed circular plasmid DNA showed by the agarose gel analysis indicate the reagent contained in the kit is not harmful to the plasmid integrity.

3. The high purity of obtained plasmid DNA is showed by the value of OD260/280 (OD260/280=1.79).

4. 20.7ug plasmid DNA was purified from 5mL of E.coli JM109 cultured overnight. Although the plasmid yield varies depending on plasmid copy number per cell, the individual insert in a plasmid, factors that affect growth of the bacterial culture, the tested kit can get the comparable plasmid yield, to some other commercial plasmid purification kits.

5. Plasmid DNA purified with the tested kit is immediately ready for use. The purified plasmid DNA is suitable for a variety of routine applications, including restriction enzyme digestion, PCR, sequencing, library screening, ligation and transformation, and transfection of robust cells. If the plasmid was eluted with nuclease-free water, it is also suitable for the in vitro transcription and translation.

6. To the average molecular biologist working with RNA, preventing, detecting, and eliminating nuclease contamination, particularly ribonuclease (RNase) contamination, is a constant and somewhat annoying challenge. The routine plasmid purification kits introduce RNaseA to remove the RNA component in plasmid sample. So, the additive RNaseA should be harmful for some downstream applications, including RNA probe synthesis for Northern Blot and in situ hybridization, in vitro transcription, and in vitro translation. As far as I know, this is the first plasmid purification kit that remove RNA component without the addition of RNaseA as a necessary.