Anti-Histone H3 Antibody, K27M mutant

是否单克隆 ：多克隆

抗体英文名 ：Anti-Histone H3 Antibody, K27M mutant

抗原来源 ：人，小鼠

应用范围 ：WB, ChIP

规格 ：100 µg

Anti-Histone H3 Antibody, K27M mutant ABE419

Anti-Histone H3 Antibody, K27M mutant, is validated for use in western blotting (WB) & Chromatin immunoprecipitation (ChIP).

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, H	WB, ChIP	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABE419
Description	Anti-Histone H3 Antibody, K27M mutant
Alternate Names	Histone H3.1 Histone H3.3
Background Information	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.

Product Information
Format	Affinity Purified
Control	Lysates from MEF transfectants expressing K27M (positive) or wildtype (negative) FLAG-HA-tagged histone H3.3.
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Anti-Histone H3 Antibody, K27M mutant, is validated for use in western blotting (WB) & Chromatin immunoprecipitation (ChIP).
Key Applications	Western Blotting Chromatin Immunoprecipitation (ChIP)
Application Notes	Western Blotting Analysis: A representative lot detected histone H3 K27M mutant in diffuse intrinsic pontice Glioma tissue lysates expressing Histone H3 K27M mutant. (Lewis, P. W., et al. (2013). Science. 340(6134):857-861. Chromatin Immunoprecipitation: A representative lot co-precipitated chromatin fragments containing the promoter regions of ACTA and CCT8 genes from HEK293T transfectants expressing FLAG-HA-tagged histone H3.3 with K27M mutation (Peter W. Lewis and David Allis, Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY).

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to sequence near the N-terminus of human Histone H3 with K27M mutation.
Epitope	Histsone H3 sequence surrounding K27M mutation
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes Histone H3 with K27M mutation.
Species Reactivity	Mouse Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_002098
Gene Symbol	H3F3A H3.3A H3F3 PP781 H3F3B H3.3B
Purification Method	Immunogen Affinity Purified
UniProt Number	P84243
Molecular Weight	~17 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in MEF (H3.3) and MEF (H3.3 K27M-HA-Flag) transfected cell lysate. Western Blotting Analysis: 0.2 µg/mL of this antibody detected FLAG-HA-tagged K27M mutant, but not wildtype, histone H3 in 3x10E5 cell equivalent of lysate from MEF transfectants.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg