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供应商 ：研卉生物
保存条件 ：低温
英文名 ：Reptile Batroxobin (rRpBX/SVTLE)
库存 ：2
规格 ：50ug
Recombinant
Reptile Batroxobin
(rRpBX/SVTLE)
PrimeGene Technical Data Sheet
Catalog Number:
4G1-01
Source:
Pichia pastoris
Molecular Weight:
A single, glycosylated, polypeptide chain containi
ng 231 amino acids, and having a molecular mass
of approximately 28,000~33,000.
Quantity:
10"g/50"g/1000"g
AA Sequence:
VIGGDECDIN EHPFLAFMYY SPRYFCGMTL INQEWVLTAA HCNRRFM
RIH
LGKHAGSVAN YDEVVRYPKE KFICPNKKKN VITDKDIMLI RLDRPVK
NSE
HIAPLSLPSN PPSVGSVCRI MGWGAITTSE DTYPDVPHCA NINLFNN
TVC
REAYNGLPAK TLCAGVLQGG IDTCGGDSGG PLICNGQFQG ILSWGS
DPCA
EPRKPAFYTK VFDYLPWIQS IIAGNKTATC P
Purity:
>
97% by SDS-PAGE and HPLC analyses.
Biological Activity:
Fully biologically active when compared to standar
d. The biologically activity of rRpBX is no less
than 500KU/mg (Klobusitzky Unit). One KU is defined
as the amount of enzyme which coagulates
standard human plasma incubated at 37 ºC in vitro w
ithin 60±20 seconds.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried)
powder.
Formulation:
Lyophilized from a 0.2
μ
m filtered concentrated sodium acetate buffer solut
ion.
Endotoxin:
Less than 0.5EU/mg of rRpBX/SVTLE as determined by
LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged
prior to opening to bring the contents to the
bottom. Reconstitute in sterile distilled water or
aqueous buffer containing 0.1% BSA to a
concentration of 0.1-1.0 mg/ml. Stock solutions sho
uld be apportioned into working aliquots and
stored at <-20
°
C. Further dilutions should be made in appropriate
buffered solutions.
Storage:
This lyophilized preparation is stable at 2-8
°
C, but should be kept at -20
°
C for long term storage,
preferably desiccated. Upon reconstitution, the pre
paration is stable for up to one week at 2-8
°
C. For
maximal stability, apportion the reconstituted prep
aration into working aliquots and store at -20
°
C to
-70
°
C.
Avoid repeated freeze/thaw cycles
.
Usage:
This material is offered by Shanghai PrimeGene Bio-
Tech for research, laboratory or further
evaluation purposes.
NOT FOR HUMAN USE
.
Reptile Batroxobin
Batroxobin is a serine protease derived from the ve
nom of Bothrops atrox. This thrombin-like proteolyt
ic enzyme splits the 16
Arg-17 Gly bond in the A (alpha)-chain of fibrinoge
n, releasing fibrinopeptide A and leading to the cl
ot formation through
aggregation of formed of fibrin I monomer or Des-AA
-monomer. In contrast to other anti-coagulants, it
does not affect the
functions of platelets. Hence, Batroxobin is used t
o determine fibrinogen in plasma, to measure a 'bat
roxobin clotting time' as a
heparin-insensitive parallel to the thrombin time,
to investigate dysfibrinogenemia, and to test the c
ontractile system of platelets.
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