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保质期 :1年
英文名 :Human Methylated & Non-methylated DNA Set
库存 :大量
供应商 :简石生物
保存条件 :-20C
规格 :组
HIGHLIGHTS
- Purified, non-methylated and methylated human DNA for use as negative and positive controls in methylation detection applications. Standards can be assayed in parallel with samples to monitor bisulfite conversion efficiency.
- Ideal controls for bisulfite sequencing PCR (BSP) and methylation specific PCR (MSP).
- Each standard is provided with a primer set to amplify a fragment of DNA after bisulfite conversion
DESCRIPTION
The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation family of products. This DNA set can be included as a positive and negative control to assess the efficiency of bisulfite conversion of DNA. The non-methylated human DNA is purified from the HCT116 DKO cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites.
简石生物技术(北京)有限公司
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入驻年限:14年