库存 ：500

供应商 ：武汉华美生物工程有限公司

检测限 ：125 pg/mL-8000 pg/mL

检测方法 ：Sandwich

应用 ：ELISA

适应物种 ：Mus musculus (Mouse)

样本 ：serum, plasma, tissue homogenates

规格 ：96T/48T

产品描述

This Mouse PLAT ELISA Kit was designed for the quantitative measurement of Mouse PLAT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

别名

Plat ELISA Kit; Tissue-type plasminogen activator ELISA Kit; t-PA ELISA Kit; t-plasminogen activator ELISA Kit; tPA ELISA Kit; EC 3.4.21.68) [Cleaved into: Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B] ELISA Kit

缩写

PLAT

Uniprot No

P11214

灵敏度

31.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of mouse t-PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		96
		Range %		93-99
1:2		Average %		104
		Range %		100-108
1:4		Average %		86
		Range %		83-90
1:8		Average %		104
		Range %		100-108

回收率

The recovery of mouse t-PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		85-93
EDTA plasma (n=4)		102		98-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 8000 2.076 2.000 2.038 1.865 4000 1.453 1.501 1.477 1.304 2000 0.947 0.938 0.943 0.770 1000 0.564 0.559 0.562 0.389 500 0.348 0.351 0.350 0.177 250 0.241 0.246 0.244 0.071 125 0.205 0.200 0.203 0.030 0 0.172 0.174 0.173

货期

3-5 working days