库存 :500
供应商 :武汉华美生物工程有限公司
检测限 :3.906 pg/mL-250 pg/mL
检测方法 :Sandwich
应用 :ELISA
适应物种 :Mus musculus (Mouse)
样本 :serum, plasma, cell culture supernates, tissue homogenates
规格 :96T/48T
产品描述
VEGFA(血管内皮生长因子A)是一种在人体中发挥重要作用的细胞因子,它促进血管新生和修复,并参与了许多生物学过程,如器官发育和肿瘤发生等。VEGFA在肿瘤治疗中也有重要的应用,如抑制血管生成来抑制肿瘤生长。
华美生物所提供的Mouse Vascular Endothelial cell Growth Factor,VEGF ELISA KIT属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、细胞培养上清、组织匀浆样本中的VEGFA,其灵敏度为0.857 pg/mL,检测范围为3.906 pg/mL-250 pg/mL。
别名
Vegfa ELISA Kit; Vegf ELISA Kit; Vascular endothelial growth factor A ELISA Kit; VEGF-A ELISA Kit; Vascular permeability factor ELISA Kit; VPF ELISA Kit
缩写
VEGFA
Uniprot No
Q00731
灵敏度
0.857 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度
To assess the linearity of the assay, samples were spiked with high concentrations of Mouse VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
货期
3-5 working days
酶联免疫试剂盒
酶联免疫检测试剂盒(ELISA kit)是一种快速、方便、准确的检测样品中靶蛋白的研究工具,被用于许多重要的研究领域,包括癌症、心血管、细胞生物学、表观遗传学、新陈代谢、免疫学、神经科学、信号转导等。
华美生物拥有16年以上的ELISA试剂盒生产经验,搭建了ELISA试剂盒开发和成熟抗原-抗体研发系统的良好平台。可提供4000多种 ELISA试剂盒,主要采用夹心法和竞争法,覆盖3000个靶标,涉及人类、小鼠、大鼠、牛等20多个种类。
华美生物的ELISA试剂盒,采用三轮质检方式,最大限度地保证了试剂盒最小的批内差、批间差。试剂盒组分经过重重优化,每个组分的稳定性都完美的经过高温破坏测验,避免了长途运输和保存过程中抗原抗体的失活。
华美生物一直致力于以合理的成本提供最高质量的ELISA试剂盒和服务,使我们的客户能够将宝贵的时间和资源用于推进自己的研究,凭借其完美的标曲、严格的质控、较高的稳定性,在世界范围内的科研试剂领域中脱颖而出,深受数十个国家上万名使用者的青睐。
产品优势
● 8大质控标准
华美生物CUSABIO ELISA试剂盒是试剂配备完整、质控严格、经过验证的即用型ELISA试剂盒。在研发的过程中,采取多个严控的步骤以保证试剂盒的卓越品质,保证提供给客户的产品具有线性、特异性、稳定性和灵敏度等的高标准。
● 涵盖20+种属
华美生物 ELISA试剂盒涵盖20多个种属,涉及多个研究领域,可满足不同客户的需求。
● 检测样本丰富
华美生物ELISA试剂盒可检测多种生物样品,包括:血清、血浆、体液如尿液、细胞培养上清液和细胞/组织裂解液等。
● 科研即用型
每个ELISA试剂盒由包被好抗体的板子和即用型试剂组成。无需特殊处理,操作简单。
● 20,000+已发表文献
华美生物ELISA试剂盒受到全球数千名研究人员的信赖,并引用到了许多优秀的高分期刊上,如:Nature,Science, Cell, Immunity, Nature Medicine, Cell Metabolism, Gastroenterology 等。
该产品被引用文献
Erythropoietin improves the efficiency of endothelial progenitor cell therapy after myocardial infarction in mice: Effects on transplanted cell survival and autologous &hellip.Cheng Y et al.Journal of Surgical Research.2012.Synthesized Multiple Antigenic Polypeptide Vaccine Based on B-Cell Epitopes of Human Heparanase Could Elicit a Potent Antimetastatic Effect on Human.Zhang J et al.PLoS One.2013.Potential Mechanism for Endothelial Progenitor Cell Therapy in Acute Myocardial Infarction: Activation of VEGF-PI3K/Akte-NOS Pathway.Cheng Y et al.Ann Clin Lab Sci.2013.Biomarkers of aging, life span and spontaneous carcinogenesis in the wild type and HER-2 transgenic FVB/N female mice.Panchenko AV. et al.Biogerontology.2015.The effects of tao-Hong-si-Wu on hepatic necroinflammatory activity and fibrosis in a murine model of chronic liver disease.Xi S. et al.J Ethnopharmacol.2016.The Effects of Taoren-Honghua Herb Pair on Pathological Microvessel and Angiogenesis-Associated Signaling Pathway in Mice Model of CCl4-Induced Chronic Liver Disease.Xi S.et al.Evidence-Based Complementary and Alternative Medicine.2016.Efek Pemberian Ekstrak Biji Jarak Pagar (Jatropha curcas L) Terhadap Kadar Protein VEGF dan Gambaran Histologi Vena Sentralis Jaringan Hepar..Nabilah Aulia Hasanuddin.et al.UIN SYARIF HIDAYATULLAH JAKARTA.2016.Efficacy of Ciji Hua'ai Baosheng formula on the expressions of vascular endothelial growth factor, kinase insert domain-containing receptor and basic fibroblast growth factor in mouse models of H22 hepatocellular carcinoma.Zhao Xinyue.et al.Journal of Traditional Chinese Medicine.2017.Taxol alleviates collagen-induced arthritis in mice by inhibiting the formation of microvessels.Xu J.et al.Clin Rheumatol..2017.LncRNA-AK131850 Sponges MiR-93-5p in Newborn and Mature Osteoclasts to Enhance the Secretion of Vascular Endothelial Growth Factor a Promoting Vasculogenesis of Endothelial Progenitor Cells.Quan H.et al.Cell Physiol Biochem.2018.Combination leflunomide and methotrexate impedes the recovery of liver fibrosis, partly through inhibition of myeloid cell admittance.Mengxin Lin, et al.Molecular Medicine Reports.2019.6-Gingerol delays tumorigenesis in benzo[a]pyrene and dextran sulphate sodium-induced colorectal cancer in mice.EO Farombi.Food and Chemical Toxicology.2020.Oleanolic Acid Suppressed DMBA-Induced Liver Carcinogenesis through Induction of Mitochondrial-Mediated Apoptosis and Autophagy.S Hosny.Nutr Cancer.2020.The U2AF2 /circRNA ARF1/miR-342–3p/ISL2 feedback loop regulates angiogenesis in glioma stem cells.Y Jiang.Journal of Experimental & Clinical Cancer Research.2020.Recapitulation of In Situ Endochondral Ossification Using an Injectable Hypoxia‐Mimetic Hydrogel.L Sun.ADVANCED FUNCTIONAL MATERIALS.2020.Construction of biological factor-coated stent and its effect on promoting endothelialization.Xiuhong Huang.Materials Science & Engineering C-Materials for Biological Applications.2021.STAT3 activation by catalpol promotes osteogenesis-angiogenesis coupling, thus accelerating osteoporotic bone repair.Yating Li.Stem Cell Research & Therapy.2021.Sphingosine 1-phosphate receptor 2 promotes erythrocyte clearance by vascular smooth muscle cells in intraplaque hemorrhage through MFG-E8 production.D Pan.Cellular signalling.2022.SIRT1/FOXO1 Axis-Mediated Hippocampal Angiogenesis is Involved in the Antidepressant Effect of Chaihu Shugan San.S Zhang.Drug design, development and therapy.2022.Atf4 regulates angiogenic differences between alveolar bone and long bone macrophages by regulating M1 polarization, based on single-cell RNA sequencing, RNA-seq and ATAC-seq analysis.L Gu.Journal of translational medicine.2023.Single-cell analysis reveals inflammatory interactions driving macular degeneration.M Kuchroo.Nature communications.2023.