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提供商 :武汉华美生物工程有限公司
服务名称 :多克隆抗体定制服务
规格 :根据实际服务情况来定价
多克隆抗体定制服务
多克隆抗体存在于免疫动物的血清中,可通过直接分离血清获得。由于具有可识别多个表位、可引起凝集反应、沉淀反应等优点,被广泛应用于免疫学诊断领域。比如经常用到的Western blotting实验中的酶标二抗和ELISA实验中的包被抗体。而且制备费用也较单克隆抗体低。
华美生物自主建立抗体研发生产平台,可为您提供可靠的多克隆抗体定制服务,并有多种免疫原和多种宿主物种可供选择。您可以选择自己提供抗原或者我们帮您设计并制备抗原,我们将提供给您满足您实验需求的多克隆抗体产品。
服务流程:
服务内容及周期:
免疫原 | 服务内容 | 交付内容 | 服务周期 |
---|---|---|---|
多肽 |
|
|
12-14 周 |
重组蛋白 | 14-16 周 | ||
天然蛋白 | 12-14 周 |
服务实例:
ELISA
Antigen coating concentration 2 ug/ml
Antiserum 1:2000 is more than diluted
Secondary:Goat polyclonal to rabbit IgG at 1/50000 dilution
Western Blot
Positive WB detected in:K562 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate,Rat liver tissue, Mouse brain tissue, Mouse lung tissue
All lanes:a antibody at 2ug/ ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64 KDa
Ob_served band size: 64 KDa
Western Blot
WT: Wild-type 293 cells
KO: Knockout 293 cells
IHC image of A antibody diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with A antibody at 1:400,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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入驻年限:13年