Anti-HSP70 Antibody (monoclonal, BRM-22)(BM0368-150ul)

价格： ￥2780

品牌：BOSTER 品牌认证

货号：BM0368-150ul

产品详情

产品概况

货号	BM0368
产品名称	Anti-HSP70/HSPA1A Antibody
基因名	HSPA1A
抗体来源	Mouse
克隆	Monoclonal(Clone:BRM-22)
抗体亚型	Mouse IgG1
分子量	70KD
免疫原	HSP70 isolated from bovine brain.
内容	200 ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA
纯化方式	Ascites
浓度	200ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex(MHC) class III region 92 kilobases(kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
研究类别	1. Milner, C. M.; Campbell, R. D. : Structure and expression of the three MHC-linked HSP70 genes. Immunogenetics 32: 242-251, 1990. 2. Ungewickell, E. : The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles. EMBO J. 4: 3385-3391, 1985.
Uniprot ID	HSPA1A: P0DMV8
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry in paraffin section (IHC):	1:50-400
Immunohistochemistry in frozen section (IHC-F):	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- HSP70/HSPA1A antibody (BM0368). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates,
Lane 2: 293T whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: U20S whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse liver tissue lysates.
Use mouse anti- HSP70/HSPA1A 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for HSP70/HSPA1A at approximately 70KD. The expected band size for HSP70/HSPA1A is at 70KD.|Figure 2. IHC analysis using anti- HSPA1A antibody (BM4403). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.[/list_product_images]