Anti-GATA3 Antibody (Clone#OTI5C11)(MA00593-150ul)

价格： ￥2780

品牌：BOSTER 品牌认证

货号：MA00593-150ul

产品详情

产品概况

货号	MA00593
产品名称	Anti-GATA3 Antibody (Clone#OTI5C11)
基因名	GATA3
抗体来源	Mouse
克隆	OTI5C11
抗体亚型	Mouse IgG2b
分子量	47.9KD
免疫原	Human recombinant protein fragment corresponding to amino acids 155-443 of human GATA3 (NP_002042) produced in E.coli.
内容	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度	500 ug/ml
产品形态	Liquid
保存条件	Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料	This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
研究类别	1. Joulin V, Bories D, Eleouet JF, Labastie MC, Chretien S, Mattei MG, Romeo PH (Jul 1991). "A T-cell specific TCR delta DNA binding protein is a member of the human GATA family"2. Kouros-Mehr Hosein, Slorach EM, Sternlicht MD, Werb Z (December 2006). "GATA-3 maintains the differentiation of the luminal cell fate in the mammary gland". Cell 127 (5): 1041–55.3. Wilson BJ (2008). "Does GATA3 act in tissue-specific pathways A meta-analysis-based approach". J. Carcinogenesis 7: 6.
Uniprot ID	GATA3: P23771

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Immunohistochemistry in paraffin section (IHC):	1:200

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00593) (1:200)|Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00593) (1:200)|Figure 3. Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00593) (1:200)|Figure 4. Flow cytometric Analysis of MCF-7 cells, using anti-GATA3 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).The right is HUVEC cells as negtive control (1:100).|Figure 5. Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA00593) (1:200)|Figure 6. Immunocytochemistry staining of MCF-7 cells using anti-GATA3 mouse monoclonal antibody (Left). The right is negative control. (1:2000)|Figure 7. Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-GATA3 monoclonal antibody (1:200).[/list_product_images]