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供应商 ：广州威佳科技有限公司
规格 ：150ul
产品概况
| 货号 | A05329-1 |
|---|---|
| 产品名称 | Anti-VAPA Antibody |
| 基因名 | VAPA |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 33KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of human VAPA (KHEQILVLDPPTDLKFK). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | VAMP-Associated Protein A ( or Vesicle-Associated Membrane Protein-Associated Protein A) is a protein that in humans is encoded by the VAPA gene. The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. |
| 研究类别 | 1. Nishimura, Y., Hayashi, M., Inada, H., Tanaka, T. Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins. Biochem. Biophys. Res. Commun. 254: 21-26, 1999.2. Weir, M. L., Klip, A., Trimble, W. S. Identification of a human homologue of the vesicle-associated membrane protein (VAMP)-associated protein of 33 kDa (VAP-33): a broadly expressed protein that binds to VAMP. Biochem. J. 333: 247-251, 1998. |
| Uniprot ID | VAPA: Q9P0L0 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- VAPA antibody (A05329-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
Use rabbit anti- VAPA 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for VAPA at approximately 33KD. The expected band size for VAPA is at 28KD.|Figure 2. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of human cervical cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of human colorectal adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of human lung adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of mouse colon tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of rat colon tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 8. IF analysis using anti- VAPA antibody (A05329-1) detected in paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1032) and counterstained with DAPI (blue).|Figure 9. IF analysis using anti- VAPA antibody (A05329-1) detected in paraffin-embedded section of rat colon tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1032) and counterstained with DAPI (blue).|Figure 10. ICC analysis using anti- VAPA antibody (A05329-1). was detected in immersion fixed PC-3 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).|Figure 11. Flow cytometry analysis of U87 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
Use rabbit anti- VAPA 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for VAPA at approximately 33KD. The expected band size for VAPA is at 28KD.|Figure 2. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of human cervical cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of human colorectal adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of human lung adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of mouse colon tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- VAPA antibody (A05329-1). detected in paraffin-embedded section of rat colon tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 8. IF analysis using anti- VAPA antibody (A05329-1) detected in paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1032) and counterstained with DAPI (blue).|Figure 9. IF analysis using anti- VAPA antibody (A05329-1) detected in paraffin-embedded section of rat colon tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1032) and counterstained with DAPI (blue).|Figure 10. ICC analysis using anti- VAPA antibody (A05329-1). was detected in immersion fixed PC-3 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).|Figure 11. Flow cytometry analysis of U87 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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