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供应商 ：广州威佳科技有限公司
规格 ：150ul
产品概况
| 货号 | A02038-3 |
|---|---|
| 产品名称 | Anti-JUN Antibody |
| 基因名 | JUN |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 36KD |
| 免疫原 | E.coli-derived human c-Jun/JUN recombinant protein (Position: K35-F331). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | c-Jun is a protein that in humans is encoded by the JUN gene. This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. |
| 研究类别 | 1. Aguilera, C., Nakagawa, K., Sancho, R., Chakraborty, A., Hendrich, B., Behrens, A. c-Jun N-terminal phosphorylation antagonises recruitment of the Mbd3/NuRD repressor complex. Nature 469: 231-235, 2011. 2. Bahary, N., Zorich, G., Pachter, J. E., Leibel, R. L., Friedman, J. M. Molecular genetic linkage maps of mouse chromosomes 4 and 6. Genomics 11: 33-47, 1991. 3. Behrens, A., Sibilia, M., Wagner, E. F. Amino-terminal phosphorylation of c-Jun regulates stress-induced apoptosis and cellular proliferation. Nature Genet. 21: 326-329, 1999. |
| Uniprot ID | JUN: P05412 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- JUN Antibody (A02038-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: Jurkat whole cell lysates,
Lane 4: U-87MG whole cell lysates.
Use rabbit anti-JUN 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for JUN at approximately 36KD. The expected band size for JUN is at 36KD.|Figure 2. IHC analysis of JUN Antibody (A02038-3). was detected in paraffin-embedded section of human renal cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of JUN Antibody (A02038-3). was detected in paraffin-embedded section of human the renal pelvis is squamous metaplasia tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. ICC analysis using Anti-JUN Antibody (A02038-3) and anti-TUBB antibody (M01857-3). was detected in immersion fixed A431 cell line. Cells were stained using theDylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127), Cells were stained using the Dylight594-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1141) .|Figure 5. Flow cytometry analysis of U2OS cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: HELA whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: Jurkat whole cell lysates,
Lane 4: U-87MG whole cell lysates.
Use rabbit anti-JUN 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for JUN at approximately 36KD. The expected band size for JUN is at 36KD.|Figure 2. IHC analysis of JUN Antibody (A02038-3). was detected in paraffin-embedded section of human renal cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis of JUN Antibody (A02038-3). was detected in paraffin-embedded section of human the renal pelvis is squamous metaplasia tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. ICC analysis using Anti-JUN Antibody (A02038-3) and anti-TUBB antibody (M01857-3). was detected in immersion fixed A431 cell line. Cells were stained using theDylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127), Cells were stained using the Dylight594-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1141) .|Figure 5. Flow cytometry analysis of U2OS cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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