Anti-NeuN/Rbfox3 Antibody(A11954-1-150ul)价格

库存：大量

供应商：广州威佳科技有限公司

规格：150ul

产品概况

货号	A11954-1
产品名称	Anti-NeuN Antibody
基因名	RBFOX3
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	46-55KD
免疫原	E.coli-derived mouse NeuN/Rbfox3 recombinant protein (Position: H28-D304).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3) is a protein that in humans is encoded by the RBFOX3 gene. This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
研究类别	1. Dredge, B. K., Jensen, K. B. NeuN/Rbfox3 nuclear and cytoplasmic isoforms differentially regulate alternative splicing and nonsense-mediated decay of Rbfox2. PLoS One 6: e21585, 2011. Note: Electronic Article. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 6/22/2016.3. Kim, K. K., Adelstein, R. S., Kawamoto, S. Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors. J. Biol. Chem. 284: 31052-31061, 2009.
Uniprot ID	RBFOX3: Q8BIF2
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:500-2000
Immunohistochemistry in paraffin section (IHC):	1:50-400
Immunofluorescence (IF):	1:50-400
ELISA:	1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- NEUN antibody (A11954-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
Use rabbit anti- NEUN 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NEUN at approximately 46-55KD. The expected band size for NEUN is at 34KD.|Figure 2. IHC analysis using anti- NEUN antibody (A11954-1). detected in paraffin-embedded section of mouse brain tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- NEUN antibody (A11954-1). detected in paraffin-embedded section of rat brain tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 4. IF analysis using anti- NEUN antibody (A11954-1). detected in paraffin-embedded section of rat brain tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1032).[/list_product_images]