Anti-IRAK4 Antibody(A01247-3-150ul)

价格： ￥2780

品牌：BOSTER 品牌认证

货号：A01247-3-150ul

产品详情

产品概况

货号	A01247-3
产品名称	Anti-IRAK4 Antibody
基因名	IRAK4
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	52KD
免疫原	E.coli-derived human IRAK4 recombinant protein (Position: N419-S460).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	IRAK-4(interleukin-1 receptor-associated kinase 4), also called REN64, in the IRAK family, is a protein kinase involved in signaling innate immune responses from Toll-like receptors. It also supports signaling from T-cell receptors. Scott(2002) mapped the REN64/IRAK4 gene to chromosome 12 based on similarity between the REN64 sequence(GenBank AF155118) and a chromosome 12 clone(GenBank AC093012). Functional analysis by Li et al.(2002) determined that IRAK4, like IRAK1 and Pelle, has auto- and cross-phosphorylation kinase activity. Precipitation and binding analyses showed weak interaction between IRAK4 and IRAK1, but IRAK4 did not interact with other IRAK family members. Overexpressed IRAK4 interacted with MYD88 and TRAF6 and activated mitogen-activated protein kinase(MAPK) and nuclear factor kappa-B(NFKB) pathways.
研究类别	1. Day, N., Tangsinmankong, N., Ochs, H., Rucker, R., Picard, C., Casanova, J.-L., Haraguchi, S., Good, R. Interleukin receptor-associated kinase (IRAK-4) deficiency associated with bacterial infections and failure to sustain antibody responses. J. Pediat. 144: 524-526, 2004. 2. Li, S., Strelow, A., Fontana, E. J., Wesche, H. IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase. Proc. Nat. Acad. Sci. 99: 5567-5572, 2002. 3. Scott, A. F. Personal Communication. Baltimore, Md. 4/25/2002.
Uniprot ID	IRAK4: Q9NWZ3
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
(ELISA)：	1:100-1000

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- IRAK4 Antibody (A01247-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates.
Use rabbit anti- IRAK4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for IRAK4 at approximately 52KD. The expected band size for IRAK4 is at 52KD.[/list_product_images]