Anti-MMP9 Antibody(原货号PB0710)(PB9669-150ul)

价格： ￥2780

品牌：BOSTER 品牌认证

货号：PB9669-150ul

产品详情

产品概况

货号	PB9669
产品名称	Anti-MMP9 Antibody
基因名	MMP9
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	78KD
免疫原	A synthetic peptide corresponding to a sequence at the C-terminus of mouse MMP-9 (641-672aa KALLFSKGRVWRFDLKSQKVDPQSVIRVDKEF), different from the related human sequence by thirteen amino acids, and from the related rat sequence by eight amino acids.
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is an enzyme that in humans is encoded by the MMP9 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
研究类别	1. Template:, 92kDa type IV collagenase).2. Yuichiro Hirose et al. (May 2008). "A Functional Polymorphism in THBS2 that Affects Alternative Splicing and MMP Binding Is Associated with Lumbar-Disc Herniation".
Uniprot ID	MMP9: P41245
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry(Paraffin-embedded Section):	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

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[list_product_images]Figure 1. Western blot analysis of Anti-MMP9 antibody (PB9669). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: NRK whole cell lysates,Lane 2: ANA-1 whole cell lysates,Lane 3: HEPA whole cell lysates,Use rabbit Anti-MMP9 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MMP9 at approximately 78KD. The expected band size for MMP9 is at 78KD.|Figure 2. IHC analysis using Anti-MMP9 antibody (PB9669) detected in paraffin-embedded section of mouse kidney tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using Anti-MMP9 antibody (PB9669) detected in paraffin-embedded section of rat liver tissue. Biotinylated goat Anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.[/list_product_images]