Anti-Cystatin C/CST3 Antibody (monoclonal, 4H8)(M00961-1-150ul)价格

库存：大量

供应商：广州威佳科技有限公司

规格：150ul

产品概况

货号	M00961-1
产品名称	Anti-CST3 Antibody (monoclonal, 4H8)
基因名	CST3
抗体来源	Mouse
克隆	Monoclonal(Clone:4H8)
抗体亚型	Mouse IgG2a
分子量	16KD
免疫原	E. coli-derived human Cystatin C recombinant protein (Position: K31-A146).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	protein G purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Cystatin C or cystatin 3, a protein encoded by the CST3 gene, is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer's disease. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and body fluids. It is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Cystatin C belongs to the type 2 cystatin gene family.
Uniprot ID	CST3: P01034
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry in paraffin section (IHC):	1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):	1:50-400
Flow cytometry (FCM):	1-3 μg/1x10⁶ cells
ELISA(Cap):	1:50-1:200
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1.ICC analysis using anti-CST3 antibody (M00961-1) was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) and counterstained with DAPI (blue).|Figure 2. Western blot analysis of anti- Cystatin C/CST3 antibody (M00961-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human COLO320 whole cell lysates,
Lane 6: human HEK293 whole cell lysates.Use mouse anti- Cystatin C/CST3 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for Cystatin C/CST3 at approximately 15KD. The expected band size for Cystatin C/CST3 is at 15KD.|Figure 3.IHC analysis using anti- Cystatin C/CST3 antibody (M00961-1). detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4.IHC analysis using anti- Cystatin C/CST3 antibody (M00961-1). detected in paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5.IHC analysis using anti- Cystatin C/CST3 antibody (M00961-1). detected in paraffin-embedded section of human glioma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6.Flow cytometry analysis of A549 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]