Anti-WNT4 Antibody(A00879-150ul)价格_品牌:BOSTER-丁香通官网

库存：大量

供应商：广州威佳科技有限公司

规格：150ul

产品概况

货号	A00879
产品名称	Anti-WNT4 Antibody
基因名	WNT4
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	39KD
免疫原	E.coli-derived human WNT4 recombinant protein (Position: K100-E274).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Wingless-type MMIV integration site family, member4, is a secreted protein that in humans is encoded by the Wnt4 gene. By fluorescence in situ hybridization, WNT4 gene was mapped to chromosome 1p35, a location consistent with the results of radiation hybrid mapping. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and embryogenesis.
研究类别	1. Biason-Lauber, A., De Filippo, G., Konrad, D., Scarano, G., Nazzaro, A., Schoenle, E. J. WNT4 deficiency--a clinical phenotype distinct from the classic Mayer-Rokitansky-Kuster-Hauser syndrome: a case report. Hum. Reprod. 22: 224-229, 2007.2. Biason-Lauber, A., Konrad, D., Navratil, F., Schoenle, E. J. A WNT4 mutation associated with mullerian-duct regression and virilization in a 46,XX woman. New Eng. J. Med. 351: 792-798, 2004.3. Garnis, C., Campbell, J., Davies, J. J., MacAulay, C., Lam, S., Lam, W. L. Involvement of multiple developmental genes on chromosome 1p in lung tumorigenesis. Hum. Molec. Genet. 14: 475-482, 2005.
Uniprot ID	WNT4: P56705
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry in paraffin section (IHC):	1:50-400
(ELISA)：	1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. IHC analysis of WNT4 using anti-WNT4 antibody (A00879).WNT4 was detected in paraffin-embedded section of human liver cancer tissues. anti-WNT4 Antibody (A00879) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 2. IHC analysis of WNT4 using anti-WNT4 antibody (A00879).WNT4 was detected in paraffin-embedded section of human renal cancer tissues. anti-WNT4 Antibody (A00879) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. Western blot analysis of WNT4 using anti-WNT4 antibody (A00879).

Lane 1: human HEK293 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human Hela whole cell lysates.
anti-WNT4 antigen affinity purified polyclonal antibody (Catalog # A00879)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for WNT4 at approximately 39KD. The expected band size for WNT4 is at 39KD.[/list_product_images]