Anti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody(PB0504-100ul)

价格： ￥1960

品牌：BOSTER 品牌认证

货号：PB0504-100ul

产品详情

产品概况

货号	PB0504
产品名称	Anti-ATP1A1 Antibody
基因名	ATP1A1
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	100-113KD
免疫原	A synthetic peptide corresponding to a sequence at the N-terminus of human ATP1A1 (7-34aa RDKYEPAAVSEQGDKKGKKGKKDRDMDE), different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
研究类别	1. "Entrez Gene: ATP1A1 ATPase, Na+/K+transporting, alpha 1 polypeptide".2. Dunbar LA, Caplan MJ (2001). "Ion pumps in polarized cells: sorting and regulation of the Na+, K+- and H+, K+-ATPases.". J. Biol. Chem. 276 (32): 29617–20.3. Xie Z, Cai T (2004). "Na+-K+--ATPase-mediated signal transduction: from protein interaction to cellular function.". Mol. Interv. 3(3): 157–68.
Uniprot ID	ATP1A1: P05023
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- ATP1A1 antibody (PB0504).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysates,Lane 2: human U87-MG whole cell lysates,Lane 3: human HEK293 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: human HEPG2 whole cell lysates,Lane 6: human SW620 whole cell lysates,Lane 7: monkey COS-7 whole cell lysates,Lane 8: human K562 whole cell lysates.Use rabbit anti- ATP1A1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP1A1 at approximately 100-113KD. The expected band size for ATP1A1 at 113KD.[/list_product_images]