产品详情
文献和实验
相关推荐
库存 ：大量
供应商 ：广州威佳科技有限公司
规格 ：50ul
产品概况
| 货号 | A06942-1 |
|---|---|
| 产品名称 | Anti-PIGS Antibody |
| 基因名 | PIGS |
| 抗体来源 | Rabbit |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 65KD |
| 免疫原 | E.coli-derived human PIGS recombinant protein (Position: R184-D504). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | GPI transamidase component PIG-S is an enzyme that in humans is encoded by the PIGS gene. This gene encodes a protein that is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. |
| 研究类别 | 1. Efthymiou, S., Dutra-Clarke, M., Maroofian, R., Kaiyrzhanov, R., Scala, M., Alvi, J. R., Sultan, T., Christoforou, M., Nguyen, T. T. M., Mankad, K., Vona, B., Rad, A., Striano, P., Salpietro, V., Guillen Sacoto, M. J., Zaki, M. S., Gleeson, J. G., Campeau, P. M., Russell, B. E., Houlden, H. Expanding the phenotype of PIGS-associated early onset epileptic developmental encephalopathy. Epilepsia 62: e35-e41, 2021.2. Gross, M. B. Personal Communication. Baltimore, Md. 7/21/2020.3. Nguyen, T. T. M., Murakami, Y., Wigby, K. M., Baratang, N. V., Rousseau, J., St-Denis, A., Rosenfeld, J. A., Laniewski, S. C., Jones, J., Iglesias, A. D., Jones, M. C., Masser-Frye, D., and 7 others. Mutations in PIGS, encoding a GPI transamidase, cause a neurological syndrome ranging from fetal akinesia to epileptic encephalopathy. Am. J. Hum. Genet. 103: 602-611, 2018. |
| Uniprot ID | PIGS: Q96S52 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry(Intracellular): | 1-3 μg/1x106 cells |
| Direct ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]Figure 1. Western blot analysis of anti-PIGS antibody (A06942-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue(HCCT) lysates,
Lane 3: human hepatocellular carcinoma paracancerous tissue(HCCP) lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat RH35 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PIGS antigen affinity purified polyclonal antibody (A06942-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PIGS at approximately 65 kDa. The expected band size for PIGS is at 62,65-70 kDa.|Figure 2. IF analysis of PIGS using anti-PIGS antibody (A06942-1).
PIGS was detected in an immunocytochemical section of Hela cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 3. Flow Cytometry analysis of RT4 cells using anti-PIGS antibody (A06942-1).
Overlay histogram showing RT4 cells stained with A06942-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIGS Antibody (A06942-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human hepatocellular carcinoma tumor tissue(HCCT) lysates,
Lane 3: human hepatocellular carcinoma paracancerous tissue(HCCP) lysates,
Lane 3: rat liver tissue lysates,
Lane 4: rat RH35 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PIGS antigen affinity purified polyclonal antibody (A06942-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PIGS at approximately 65 kDa. The expected band size for PIGS is at 62,65-70 kDa.|Figure 2. IF analysis of PIGS using anti-PIGS antibody (A06942-1).
PIGS was detected in an immunocytochemical section of Hela cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).|Figure 3. Flow Cytometry analysis of RT4 cells using anti-PIGS antibody (A06942-1).
Overlay histogram showing RT4 cells stained with A06942-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIGS Antibody (A06942-1, 1 μg/1x106 cells). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
广州威佳科技有限公司
代理商实名认证
金牌会员
入驻年限：11年
