Anti-HGD Antibody (monoclonal, 2F11E1)(M01909-1-50ul)

价格： ￥1180

品牌：BOSTER 品牌认证

货号：M01909-1-50ul

产品详情

产品概况

货号	M01909-1
产品名称	Anti-HGD Antibody (monoclonal, 2F11E1)
基因名	HGD
抗体来源	Mouse
克隆	Monoclonal(Clone:2F11E1)
抗体亚型	Mouse IgG2b
分子量	50KD
免疫原	E. coli-derived human HGD recombinant protein (Position: D374-N445).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	protein G purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	The HGD gene encodes homogentisate 1,2-dioxygenase (HGD), an enzyme involved in the catabolism of phenylalanine and tyrosine. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria. This gene is mapped to chromosome 3q21-q23 by a preliminary PCR screen of hamster/human somatic cell hybrid genomic DNA samples and by fluorescence in situ hybridization.
研究类别	1. Fernandez-Canon, J. M., Granadino, B., Beltran-Valero de Bernabe, D., Renedo, M., Fernandez-Ruiz, E., Penalva, M. A., Rodriguez de Cordoba, S. The molecular basis of alkaptonuria. Nature Genet. 14: 19-24, 1996.2. Vilboux, T., Kayser, M., Introne, W., Suwannarat, P., Bernardini, I., Fischer, R., O'Brien, K., Kleta, R., Huizing, M., Gahl, W. A. Mutation spectrum of homogentisic acid oxidase (HGD) in alkaptonuria. Hum. Mutat. 30: 1611-1619, 2009.
Uniprot ID	HGD: Q93099
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot (WB):	1:500-2000
Immunohistochemistry(Paraffin-embedded Section):	1:50-400
Flow cytometry (FCM):	1-3 μg/1x10⁶ cells
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

点击图片放大

[list_product_images]Figure 1. Western blot analysis of anti- HGD Antibody (M01909-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HCCP tissue lysates.
Use mouse anti- HGD 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for HGD at approximately 50KD. The expected band size for HGD is at 50KD.|Figure 2. IHC analysis using anti- HGD Antibody (M01909-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog#SV0001) with DAB as the chromogen.|Figure 3. Flow cytometry analysis of CACO-2 cell (1x106) DyLight 488 conjugated goat anti- mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]