Anti-MAP2K2 Antibody(A00996-3-50ul)

价格： ￥1180

品牌：BOSTER 品牌认证

货号：A00996-3-50ul

产品详情

产品概况

货号	A00996-3
产品名称	Anti-MAP2K2 Antibody
基因名	MAP2K2
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	45KD
免疫原	E.coli-derived human MEK2/MAP2K2 recombinant protein (Position: M1-E45).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Dual specificity mitogen-activated protein kinase kinase 2(MAP2K2), also called PRKMK2 or MEK2, is an enzyme that in humans is encoded by the MAP2K2 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. MAP2K2 is mapped to 19p13.3. This kinase is known to play a critical role in mitogen growth factor signal transduction, and the inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax. Recombinant MEK2 and MEK1 both could activate human ERK1 in vitro, and they further characterized biochemically the 2 MAP2Ks. MAP2K2 has been shown to interact with MAPK3 and ARAF.
研究类别	1.“Entrez Gene: MAP2K2 mitogen-activated protein kinase kinase 2"2. Zheng, C. F., Guan, K. L. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J. Biol. Chem. 268: 11435-11439, 1993.
Uniprot ID	MAP2K2: P36507
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
ELISA:	1:100-1000
Flow Cytometry (FCM):	1-3 μg/1x10⁶ cells

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- MEK2/MAP2K2 Antibody (A00996-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: Jurkat whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain whole cell lysates,
Lane 6: mouse lung tissue lysates.
Use rabbit anti- MAP2K2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MAP2K2 at approximately 44KD. The expected band size for MAP2K2 is at 44KD.|Figure 2. Flow cytometry analysis of RH35 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]