Anti-SLC44A2 Antibody(A05467-1-50ul)

价格： ￥1180

品牌：BOSTER 品牌认证

货号：A05467-1-50ul

产品详情

产品概况

货号	A05467-1
产品名称	Anti-SLC44A2 Antibody
基因名	Slc44a2
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	80KD
免疫原	E.coli-derived mouse SLC44A2 recombinant protein (Position: M1-S706).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Choline transporter-like protein 2 is a protein that in humans is encoded by the SLC44A2 gene. CTL2 is a multi-pass membrane protein expressed in cells of the inner ear. CTL2 is a possible candidate for autoimmune hearing loss in humans. Present in supporting cells of the inner ear (at protein level). Only isoform 3 is expressed in inner ear vestibular tissue.
研究类别	1. Greinacher, A., Wesche, J., Hammer, E., Furll, B., Volker, U., Reil, A., Bux, J. Characterization of the human neutrophil alloantigen-3a. Nature Med. 16: 45-48, 2010. 2. O'Regan, S., Traiffort, E., Ruat, M., Cha, N., Compaore, D., Meunier, F.-M. An electric lobe suppressor for a yeast choline transport mutation belongs to a new family of transporter-like proteins. Proc. Nat. Acad. Sci. 97: 1835-1840, 2000.
Uniprot ID	Slc44a2: Q8BY89
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunohistochemistry in paraffin section IHC	1:50-400
Flow cytometry (FCM):	1-3 μg/1x10⁶ cells
(ELISA)：	1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- SLC44A2 antibody (A05467-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse lung tissue lysates.Use rabbit anti- SLC44A2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SLC44A2 at approximately 80KD. The expected band size for SLC44A2 is at 80KD.|Figure 2. IHC analysis using anti- SLC44A2 antibody (A05467-1). detected in paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. Flow cytometry analysis of HEPA1-6 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]