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库存 :大量
供应商 :广州威佳科技有限公司
规格 :50ul
产品概况
货号 | M00065-1 |
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产品名称 | Anti-CD28 Antibody (monoclonal, AGOA-3) |
基因名 | CD28 |
抗体来源 | Mouse |
克隆 | Monoclonal(Clone:AGOA-3) |
抗体亚型 | Mouse IgG1, κ |
免疫原 | A20 cells expressing mouse CD28 and a recombinant mouse CD28-Ig fusion protein |
内容 | 200 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | protein G purified. |
浓度 | 200 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co- stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). The CD28 gene is mapped to 2q33-q34. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. |
Uniprot ID | CD28: P31041 |
推荐配套的二抗和检测试剂 | Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC. |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. IHC analysis of CD28 using anti- CD28 antibody (M00065-1). detected in paraffin-embedded section of mouse thymus tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.[/list_product_images]
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入驻年限:11年