Anti-p38 gamma/MAPK12 Antibody(A03942-1-50ul)

价格： ￥1180

品牌：BOSTER 品牌认证

货号：A03942-1-50ul

产品详情

产品概况

货号	A03942-1
产品名称	Anti-p38 gamma/MAPK12 Antibody
基因名	MAPK12
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	42KD
免疫原	E.coli-derived human MAPK12 recombinant protein (Position: S229-L367).
内容	500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式	Immunogen affinity purified.
浓度	500 ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料	Mitogen-activated protein kinase 12 (MAP kinase 12), also known as extracellular signal-regulated kinase 6 (ERK6) or stress-activated protein kinase 3 (SAPK3), is an enzyme that in humans is encoded by the MAPK12 gene. Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes.
研究类别	1. Goedert, M., Hasegawa, J., Craxton, M., Leversha, M. A., Clegg, S. Assignment of the human stress-activated protein kinase-3 gene (SAPK3) to chromosome 22q13.3 by fluorescence in situ hybridization. Genomics 41: 501-502, 1997. 2. Lechner, C., Zahalka, M. A., Giot, J.-F., Moller, N. P. H., Ullrich, A. ERK6, a mitogen-activated protein kinase involved in C2C12 myoblast differentiation. Proc. Nat. Acad. Sci. 93: 4355-4359, 1996. 3. Li, Z., Jiang, Y., Ulevitch, R. J., Han, J. The primary structure of p38-gamma: a new member of p38 group of MAP kinases. Biochem. Biophys. Res. Commun. 228: 334-340, 1996.
Uniprot ID	MAPK12: P53778
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC.

产品应用细节

为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot(WB):	1:500-2000
Immunocytochemistry/Immunofluorescence (ICC/IF):	1:50-400
Flow cytometry (FCM):	1-3 μg/1x10⁶ cells
(ELISA)：	1:100-1000

*稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片描述

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[list_product_images]Figure 1. Western blot analysis of anti- p38 gamma/MAPK12 Antibody (A03942-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: Hek293 whole cell lysates,
Lane 3: COS-7 whole cell lysates,
Lane 4: K562 whole cell lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: muscle skeletal muscle tissue lysates.
Use rabbit anti- MAPK12 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MAPK12 at approximately 42KD. The expected band size for MAPK12 is at 42KD.|Figure 2. ICC analysis using anti- p38 gamma/MAPK12 Antibody (A03942-1). was detected in immersion fixed HELA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).|Figure 3. Flow cytometry analysis of U20S cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]