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库存 ：大量
供应商 ：广州威佳科技有限公司
规格 ：50ul
产品概况
| 货号 | BM4543 |
|---|---|
| 产品名称 | Anti-ATG9A Antibody |
| 基因名 | ATG9A |
| 抗体来源 | Rabbit |
| 克隆 | Monoclonal |
| 抗体亚型 | Rabbit |
| 分子量 | 100-110KD |
| 免疫原 | A synthesized peptide derived from human ATG9A |
| 内容 | 500 ug/ml;Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 纯化方式 | Affinity-chromatography |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Autophagy-related protein 9A is a protein that in humans is encoded by the ATG9A gene. ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion. |
| 研究类别 | 1. Yamada, T., Carson, A. R., Caniggia, I., Umebayashi, K., Yoshimori, T., Nakabayashi, K., Scherer, S. W. Endothelial nitric-oxide synthase antisense (NOS3AS) gene encodes an autophagy-related protein (APG9-like2) highly expressed in trophoblast. J. Biol. Chem. 280: 18283-18290, 2005. |
| Uniprot ID | ATG9A: Q7Z3C6 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:20-100 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:20-100 |
| Immunocoprecipitation: | 1:20 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- ATG9A Antibody (BM4543). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HepG2 whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: HEK293 whole cell lysates,
Lane 4: HELA whole cell lysates.
Use rabbit anti- ATG9A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATG9A at approximately 100KD. The expected band size for ATG9A is at 94KD.[/list_product_images]
Lane 1: HepG2 whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: HEK293 whole cell lysates,
Lane 4: HELA whole cell lysates.
Use rabbit anti- ATG9A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATG9A at approximately 100KD. The expected band size for ATG9A is at 94KD.[/list_product_images]
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