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库存 :大量
供应商 :广州威佳科技有限公司
规格 :1ml
产品概况
货号 | BM0201 |
---|---|
产品名称 | Anti-BrdU Antibody |
抗体来源 | Mouse |
克隆 | Monoclonal |
抗体亚型 | Mouse IgG |
免疫原 | A synthetic peptide |
内容 | 200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 200ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blot(WB): | 1:500-2000 |
Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
Immunocytochemistry in fixed cells (ICC): | 1:50-400 |
Immunohistochemistry in frozen section (IHC-F): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1.IHC analysis using anti-BrdU antibody (BM0201). detected in paraffin-embedded section of HELA cells. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
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入驻年限:11年