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库存 :大量
供应商 :广州威佳科技有限公司
规格 :1ml
产品概况
货号 | M01263-4 |
---|---|
产品名称 | Anti-β-Actin Antibody |
基因名 | ACTB |
抗体来源 | Mouse |
克隆 | Monoclonal(Clone:17A10) |
抗体亚型 | Mouse IgG1 |
分子量 | 42KD |
免疫原 | A synthesized peptide derived from human beta Actin |
内容 | Mouse IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.5mg/ml BSA. |
纯化方式 | Affinity-chromatography |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. |
Uniprot ID | ACTB: P60709 |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
---|---|
Western blot (WB): | 1:3000-10000 |
IHC : | 1:100-1:200 |
ICC/IF: | 1:100-200 |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of beta Actin expression in (1) Hela cell lysate; (2) Rat brain lysate; (3) Mouse brain lysate; (4) Chicken lung lysate; (5) Rabbit testis lysate with beta Actin mouse mAb.| Figure 2. Immunohistochemical analysis of paraffin-embedded human heart, using beta Actin Mouse Monoclonal Antibody.| Figure 3. Immunofluorescent analysis of HepG2 cells, using beta Actin mAb.[/list_product_images]
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入驻年限:11年