IDH2(R140Q)

供应商 ：武汉天德

库存 ：999

靶点 ：R140Q

级别 ：科研级

目录编号 ：TD-26165

克隆性 ：单克隆

抗原来源 ：蛋白

保质期 ：24个月

抗体英文名 ：Anti IDH2(R140Q) Mouse Monoclonal Antibody

抗体名 ：IDH2(R140Q)

标记物 ：FITC

宿主 ：Mouse

适应物种 ：脊椎动物

免疫原 ： A synthetic peptide from the internal region of IDH2 which includes the mutation of R140Q, human origin.

亚型 ：lgG

形态 ：液体

应用范围 ：ELISA, WB, IF, IHC

保存条件 ：-20℃

浓度 ：1mg/ml

规格 ：100μl

IDH2(R140Q)

Cat. #::  TD-26165

Gene Symbol:  IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH

Description:   Anti-IDH2(R140Q) Mouse Monoclonal Antibody

Background:   Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

Immunogen:   A synthetic peptide from the internal region of IDH2 which includes the mutation of R140Q, human origin.

Applications:   ELISA, WB, IF, IHC

Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100

Concentration:   1 mg/ml

Host Species:   Mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:

Purity:   Purified from ascites

Preservative: No

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Recognizes R140Q mutant, but not wild type IDH2 of vertebrates.

Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

Western blot analysis of recombinant IDH2(R140Q) and wild type proteins.

Purified His-tagged IDH2(R140Q) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with anti IDH2(R140Q) monoclonal antibody (Cat. #TD-26165).

Immunofluorescence:  
 

Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R140Q) antibody.

HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R140Q) plasmid (right column), then fixed and stained with anti-IDH2(R140Q) monoclonal antibody (Cat. #TD-26165).