Mouse SLC27A4 / FATP4 ELISA Kit (A311132)

Mouse SLC27A4 / FATP4 ELISA Kit (A311132)

价格: ¥7160

品牌:Antibodies.com

货号:A311132

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检测限 :9.51 ng/L

检测方法 :Colorimetric

适应物种 :Mouse

标记物 :150-2400 ng/L

样本 :Serum, plasma or other biological fluids.

规格 :96T

Mouse SLC27A4 / FATP4 ELISA Kit (A311132) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse SLC27A4 / FATP4 in serum, plasma or other biological fluids. An antibody specific for SLC27A4 / FATP4 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the SLC27A4 / FATP4 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-SLC27A4 / FATP4 Antibody, which also binds the SLC27A4 / FATP4 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-SLC27A4 / FATP4 Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-SLC27A4 / FATP4 Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of SLC27A4 / FATP4 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of SLC27A4 / FATP4 in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

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