Human Eph Receptor B1 / NET ELISA Kit (A310271)

检测限 ：15.08 ng/L

检测方法 ：Colorimetric

适应物种 ：Human

标记物 ：300-4800 ng/L

样本 ：Serum, plasma or other biological fluids.

规格 ：96T

Human Eph Receptor B1 / NET ELISA Kit (A310271) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Eph Receptor B1 / NET in serum, plasma or other biological fluids. An antibody specific for Eph Receptor B1 / NET has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Eph Receptor B1 / NET present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-Eph Receptor B1 / NET Antibody, which also binds the Eph Receptor B1 / NET present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-Eph Receptor B1 / NET Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-Eph Receptor B1 / NET Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Eph Receptor B1 / NET captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Eph Receptor B1 / NET in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.