Human Terminal Complement Complex C5b-9 ELISA Kit (A319459)

检测限 ：7.5 ng/ml

检测方法 ：Colorimetric

适应物种 ：Human

标记物 ：12.5-800 ng/ml

样本 ：Serum, plasma, tissue homogenates, and other biological fluids.

规格 ：96T

Human Terminal Complement Complex C5b-9 ELISA Kit (A319459) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Terminal Complement Complex C5b-9 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Terminal Complement Complex C5b-9 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Terminal Complement Complex C5b-9 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Terminal Complement Complex C5b-9 Antibody, which binds the captured Terminal Complement Complex C5b-9 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Terminal Complement Complex C5b-9 captured in each well. The concentration of Terminal Complement Complex C5b-9 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.