Human BDNF ELISA Kit (A313321)

检测限 ：0.023 ng/ml

检测方法 ：Colorimetric

适应物种 ：Human

标记物 ：0.4-6.4 ng/ml

样本 ：Serum, plasma or other biological fluids.

规格 ：96T

Human BDNF ELISA Kit (A313321) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human BDNF in serum, plasma or other biological fluids. An antibody specific for BDNF has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the BDNF present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-BDNF Antibody, which also binds the BDNF present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-BDNF Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-BDNF Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of BDNF captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of BDNF in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.