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α-Lytic protease

CAS No. : 37288-76-9

MCE 国际站：α-Lytic protease

产品活性：α-Lytic protease (alphaLP) 是一种丝氨酸内肽酶。α-Lytic protease 可以在丙氨酸，丝氨酸，苏氨酸和缬氨酸氨基酸残基的羧基端侧裂解底物。

研究领域：Metabolic Enzyme/Protease

作用靶点：Ser/Thr Protease

In Vitro: Protocol
1) Combine 2 μg substrate and 50 mM ammonium bicarbonate pH 8.5 to make a 10 μL total reaction volume.
2) Add α-Lytic Protease in a 1:20 – 1:100 (α-Lytic Protease : substrate) ratio by mass.
Note: For example, add 20-100ng α-Lytic Protease to 2 μg substrate protein.
3) Incubate at 37℃ for 1 hour.
Note: longer incubations (18 hours) or more enzyme may be required, especially at lower temperatures, for complete digestion.

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