文献支持Rabbit anti-GAPDH Recombinant Monoclonal Antibody(S-240-147)
价格： ￥900
产品详情
文献和实验
相关推荐
供应商 ：爱必信(上海)生物科技有限公司
库存 ：现货
靶点 ：-
级别 ：-
目录编号 ：-
克隆性 ：-
抗原来源 ：-
保质期 ：Store at -20℃ for one year.
抗体英文名 ：Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH
抗体名 ：GAPDH抗体
标记物 ：-
宿主 ：Rabbit
适应物种 ：Human, Mouse, Rat, Zebrafish
免疫原 ：Synthetic peptide
亚型 ：-
形态 ：液态
应用范围 ：WB: 1:10000-50000, IHC-P: 1:5000
保存条件 ：Store at -20℃ for one year.
浓度 ：1mg/mL
规格 ：100uL
| 概述 | |
| 描述 | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) iswell known as one of the key enzymes involved in glycolysis.As well as functioning as a glycolytic enzyme in cytoplasm,recent evidence suggests that mammalian GAPDHis also involved in a great number of intracellular procesessuch as membrane fusion, microtubule bundling, phosphotransferaseactivity, nuclear RNA export, DNA replication,and DNA repair. |
| 别名 | GAPDH抗体;Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH |
| 宿主 | Rabbit |
| 特异性 | GAPDH antibody detects endogenous levels of total GAPDH. |
| 反应种属 | Human, Mouse, Rat, Zebrafish |
| 预测反应种属 | Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine |
| 应用 | WB: 1:10000-50000, IHC-P: 1:5000 |
| 免疫原 | Synthetic peptide |
| 性能 | |
| 形式 | Liquid |
| 浓度 | 1 mg/mL |
| 纯化方法 | Protein A affinity column |
| 类型 | Monoclonal Antibody |
| 克隆号 | S-240-147 |
| 储存/保存方法 | Store at -20℃ for one year. |
| 存储溶液 | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| 靶标 | |
| 背景说明 | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme of about 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules [PMID: 17072346]. GAPDH comprises a polypeptide chain of 335 amino acids. Structural studies identified two regions, namely the glyceraldehyde-3-phosphate catalytic site and the NAD+ binding site, a primary structure known as the Rossmann fold, which is also required for the activity of other dehydrogenases. Many of these roles are dependent on the ability of GAPDH to bind different macromolecules in the cell. |
| 翻译后修饰 | S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity.ISGylated.Sulfhydration at Cys-152 increases catalytic activity.Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation. |
| 细胞定位 | Cytoplasm, Nucleus, Membrane |
| UniProt | P04406, P16858 |
实验结果图：
WB result of GAPDH Rabbit mAb Primary antibody: GAPDH Rabbit mAb at 1/10000 dilution Lane 1: HeLa whole cell lysate 20 ug Lane 2: THP-1 whole cell lysate 20 ug Lane 3: MCF7 whole cell lysate 20 ug Lane 4: NIH/3T3 whole cell lysate 20 ug Lane 5: mouse spleen lysate 20 ug Lane 6: C6 whole cell lysate 20 ug Lane 7: rat spleen lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa Observed MW: 37 kDa Exposure time: 180 s
WB result of GAPDH Rabbit mAb Primary antibody: GAPDH Rabbit mAb at 1/50000 dilution Lane 1: HeLa whole cell lysate 20 ug Lane 2: THP-1 whole cell lysate 20 ug Lane 3: MCF7 whole cell lysate 20 ug Lane 4: NIH/3T3 whole cell lysate 20 ug Lane 5: mouse spleen lysate 20 ug Lane 6: C6 whole cell lysate 20 ug Lane 7: rat spleen lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa Observed MW: 37 kDa Exposure time: 180 s
WB result of GAPDH Rabbit mAb Primary antibody: GAPDH Rabbit mAb at 1/10000 dilution Lane 1: zebrafish lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa Observed MW: 37 kDa Exposure time: 60 s
WB result of GAPDH Rabbit mAb Primary antibody: GAPDH Rabbit mAb at 1/50000 dilution Lane 1: zebrafish lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa Observed MW: 37 kDa Exposure time: 60 s
爱必信(上海)生物科技有限公司
品牌商实名认证
钻石会员
入驻年限：12年
