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抗体名 :AbFlex®组蛋白H3ac(pan-acetyl)抗体(rAb)
抗体英文名 :AbFlex® Histone H3ac (pan-acetyl) antibody (rAb)
应用范围 :WB
宿主 :Rabbit
供应商 :Active Motif
库存 :大量
级别 :WB级抗体
保质期 :1年以上
适应物种 :Human, Wide Range Predicted
保存条件 :-20度
形态 :液态冻存
亚型 :IgG
免疫原 :This Histone H3 acetyl antibody was raised against an acetylated Histone H3 peptide.
规格 :100 µg/10 µg
Application Notes
Applications Validated by Active Motif:WB: 0.5µg/mL-2µg/mL
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
Immunogen
This Histone H3 acetyl antibody was raised against an acetylated Histone H3 peptide.Background
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
AbFlex® Histone H3ac (pan-acetyl) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.
Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histones is often associated with transcriptional activation.
获得完整的抗体列表及应用数据请浏览: www.activemotif.com/abs
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入驻年限:8年