Influenza A H7N9 (A/Shanghai/4664T/2013) Hemagglutinin / HA Protein (His Tag)

保存条件 ：Stable for 1 year at -5313°C or below from date of shipment.

保质期 ：1-2年

供应商 ：安诺伦（北京）生物科技有限公司

规格 ：100 ug

蛋白长度：A DNA sequence encoding the Influenza A virus (A/Shanghai/4664T/2013(H7N9)) hemagglutinin (AGI60292.1) (Met1-Val524) was expressed with a C-terminal polyhistidine tag.

性质：

表达系统：Baculovirus-Insect Cells

种属：H7N9

氨基酸序：Met1-Val524

标签类型：C-His

分子量：The recombinant hemagglutinin of Influenza A virus (A/Shanghai/4664T/2013(H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.6 kDa. The apparent molecular mass of the protein is approximately 58 kDa in SDS-PAGE under reducing conditions.

活性：1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.6 μg/mL for 1% GRBC.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：Harvey rat sarcoma viral oncogene homolog

通用名：HA

蛋白序参考：AGI60292.1

总结：The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile 20 mM Tris, 500 mM Nacl, 10 % glycerol, pH 8.0.Please contact us for any concerns or special requirements.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.Please refer to the specific buffer information in the hard copy of CoA.

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