文献支持M1007-11·HRP Conjugated Mouse anti-Human IgG light chain(kappa chain) Antibody [A11-G11]

靶点 ：Mouse anti-Human IgG light chain(kappa chain)

浓度 ：2 mg/mL.

应用范围 ：WB,ELISA

适应物种 ：Human

保质期 ：1年

供应商 ：Huabio

标记物 ：HRP-conjugated

保存条件 ：Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

形态 ：Liquid

亚型 ：IgG2a

免疫原 ：Human IgG light chain

规格 ：50μl/100μl

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.