文献支持细胞色素C(牛心) 9007-43-6

保存条件 ：RT/2-8℃/-20℃

保质期 ：3年

英文名 ：Cytochrome C

库存 ：999

供应商 ：北京康瑞纳生物科技有限公司

CAS号 ：9007-43-6

规格 ：25mg

细胞色素C(牛心) 9007-43-6 细胞色素C(牛心) 9007-43-6
产品名称：细胞色素C(牛心) 9007-43-6
产品编号：A1657
中文别称 肌血红质，牛心
英文别称 Cytochromes C
CAS 9007-43-6
分子式 C42H52FeN8O6S2
储存条件 -20℃
有效期 2年
溶解性 2mg/ml in water
来源 From bovine heart
纯度 min.95% as based on Mol.Wt.12,327
性状深红棕色结晶性冻干粉末
包装 瓶
规格 25mg
Product Description Cytochrome c is an electron-carrying mitochondrial protein composed of 104 amino acid residues. It is a heme protein containing a single polypeptide chain and a single heme group, which is covalently attached to the polypeptide. The ready fluctuation of cytochrome c within the cell between the ferrous and ferric states, makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen. This cytochrome c product is prepared from bovine heart by a modification of a published method.The product is supplied as a lyophilized powder. The final step before lyophilization is extensive dialysis against 6 mM ammonium hydroxide, which is volatile under lyophilization conditions, so the final product should not contain any buffer salts. The product is mainly the oxidized form of the protein. The reduced form of cytochrome c can be prepared with either sodium dithionite or sodium ascorbate, followed by gel filtration. Molecular mass:12,327 Da Isoelectric point (pI):range of 10.0 – 10.5 Spectral properties:λmax = 550 nm (reduced form) E mM = 28.0 (reduced form, 0.1 M phosphate buffer, pH 6.8) E mM = 8.4 (oxidized form, 0.1 M phosphate buffer, pH 6.8) Purity: ≥95% (SDS-PAGE and spectral assay) Preparation Instructions Cytochrome c is soluble in water or buffered solutions at pH 7.0±1.0 (up to 200 mg/ml), yielding a clear, dark red solution. For general purposes reconstitution is done at 10 mg/ml. Storage/Stability Store cytochrome c at –20 °C. The product, as supplied, is stable for 5 years. Recommended storage time for aqueous solutions: Storage at –20°C (freezer) – 6 months;Storage at 2-8°C (refrigerator) – 2 weeks ;Storage at 20-25°C (ambient temperature) – 3 days. Precautions and Disclaimer For Laboratory Use Only. Not for drug, household or other uses.

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