日本同仁 CK-04-500T CCK-8试剂盒

Cell Counting Kit-8是用于化学药物等对细胞增殖或毒性实验时进行细胞计数的试剂盒。试剂盒中使用高灵敏度的新型水溶性四唑盐WST-8甲臜染料作为显色底物，与传统的细胞计数试剂盒相比，灵敏度大幅度的提高。WST-8被细胞内的脱氢酶还原产生水溶性的甲臜染料。通过直接测量甲臜染料（450 nm）的吸光度，即可测出活细胞的数量。细胞数量与甲臜染料成正比关系。同时试剂盒为配置好的1瓶溶液，易于使用。

Cell Counting Kit-8 is a kit used for cell counting in cell proliferation or toxicity experiments such as chemical drugs. The reagent kit uses a highly sensitive new water-soluble tetrazolium salt WST-8 formazane dye as the chromogenic substrate, which significantly improves sensitivity compared to traditional cell counting kits. WST-8 is reduced by intracellular dehydrogenase to produce water-soluble formazane dye. The number of living cells can be measured by directly measuring the absorbance of the methylzan dye (450 nm). The number of cells is directly proportional to the amount of formazine dye. At the same time, the reagent kit is a prepared bottle of solution, which is easy to use.

1) Compared to the [3H] method, no radioactive isotopes are required.

2) The use of water-soluble tetrazolium salts and water-soluble formazane dyes eliminates the need for DMSO liquid exchange procedures. (Required for MTT analysis, etc.)

3) Compared with other water-soluble tetrazolium salt methods (XTT, MTS), it has higher sensitivity and lower toxicity.

4) The reagent kit is packaged in 1 bottle, without the need for advance configuration or preparation of other reagents.

5) The reagent kit has high stability.

6) The reagent can be used simultaneously with phenol red.