NFκB p65 Mouse mAb(14H2)

供应商 ：FLIGHT

库存 ：50μl/100μg

靶点 ：Monoclonal

级别 ：/

目录编号 ：FLD-M-114

克隆性 ：单克隆

抗原来源 ：/

保质期 ：1year

抗体英文名 ：NFκB p65 Mouse mAb(14H2)

抗体名 ：NFκB p65 Mouse mAb(14H2)

标记物 ：/

宿主 ：鼠

适应物种 ：H,M,R

免疫原 ：/

亚型 ：/

形态 ：液体

应用范围 ：WB、IHC、IP

保存条件 ：Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

浓度 ：100μg/瓶

规格 ：100μl/50μl

Applications: WB, IHC, IP	Reactivity: H,M,R
MW.(KDa): 65	Isotype: IgG1

Applications:          WB-Western blot     IHC-Immunochemistry       IF-Immunofluorescence     IP-Immunoprecipitation     ChIP-Chormatin Immunoprecipitation

Reactivity:
H-Human  R-Rat  M-Mouse  Mk-Monkey  Dg-Dog  Ch-Chicken  Hm-Hamster  Rb-Rabbit  Sh-Sheep  Pg-Pig

NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.

Western Blot：

Western blot analysis of 1) Hela, 2)293T,3)HepG with NFκB p65 mAb diluted at 1:2,000.

Immunohistochemistry：

IHC staining of mouse hippocampus tissue with NFκB p65 mouse mAb(14H2) diluted at 1:200.


 
Western Blot：

Western blot analysis of 1) Mouse Heart, 2)Rat Heart,3)Rat Liver, 4) Rat Spleen with NFκB p65 mAb diluted at 1:3,000.

IP：

1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis: primary antibody : FLD-M-114     1:2,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000