文献支持FOXO Reporter Kit (PI3K/AKT Pathway)

保存条件 ：-20°C

保质期 ：Stable at least 12 months from date of receipt, when stored as directed (-20°C)

英文名 ：FOXO Reporter Kit (PI3K/AKT Pathway)

库存 ：1

供应商 ：BPS Bioscience Inc.

CAS号 ：/

规格 ：500 rxns.

The FOXO Reporter kit is designed to monitor activity of the PI3K/AKT signaling pathway and the transcriptional activity of FOXO proteins in cultured cells. The kit contains the transfection-ready FOXO3 expression vector and the FOXO luciferase reporter vector, which is a PI3K/Akt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimers of the FOXO responsive element located upstream of a minimal promoter. The FOXO reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for the transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector also contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.