文献支持SRE Reporter Kit (MAPK/ERK Signaling Pathway )

保存条件 ：-20°C

保质期 ：Stable at least 12 months from date of receipt, when stored as directed (-20°C)

英文名 ：SRE Reporter Kit (MAPK/ERK Signaling Pathway )

库存 ：1

供应商 ：BPS Bioscience Inc.

CAS号 ：/

规格 ：500 rxns.

The SRE Reporter Kit is designed for monitoring the activity of the ERK signaling pathway and the transcriptional activity of SRF in cultured cells. The kit contains a transfection-ready SRE luciferase reporter vector, which is an ERK pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized SRE responsive elements located upstream of a minimal promoter. The SRE reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.