BioLegend 773104 Recombinant Human IL-21R (carrier-free)

库存 ：99

英文名 ：Recombinant Human IL-21R (carrier-free)

供应商 ：北京云肽生物科技有限公司

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BioLegend 773104 Recombinant Human IL-21R (carrier-free)
产品简介：
产品品牌：BioLegend
产品货号：773104
产品名称：BioLegend 773104 Recombinant Human IL-21R (carrier-free)
产品规格：25 µg

产品说明：
Regulatory Status   RUO
Other Names
Interleukin-21 receptor, IL-21 receptor, IL-21R, novel interleukin receptor, NILR, CD360

描述
IL-21R是一种I型跨膜蛋白，属于I类细胞因子受体家族。IL-21R含有WSXWS基序，与IL-2Rβ和IL-4Rα非常相似。IL-21R的细胞内结构域包括类似于IL-9R细胞内区域的Box 1和Box 2元件。IL-21R在B细胞上表达，在NK细胞和T细胞上以不同水平表达，但在激活后上调。一些研究表明，在一些白血病和淋巴瘤中IL-21R水平较高。IL-21R的表达也通过CD40的触发而升高。IL-21R与共同的γ亚基（CD132）形成异二聚体，该亚基由其他白细胞介素的受体共享，包括IL-2、IL-4、IL-7、IL-9和IL-15。在IL-21连接后，IL-21R与JAK1结合并诱导磷酸化级联和STAT1，3，5转导途径。一些证据表明，IL-21/IL-21R信号传导对B、NK和T细胞的增殖和分化至关重要。在正常B细胞中，IL-21介导细胞增殖、生长停滞和终末分化或凋亡，这取决于它们的激活状态。此外，缺乏IL-21R的小鼠增加了血清IgE的产生，但降低了IgG1。免疫后，抗原特异性IgG的产生受损，表明IL-21/IL-21R对调节免疫球蛋白的产生至关重要。尽管IL-21R缺乏小鼠的B细胞发育和急性CD8+T细胞反应正常，但在慢性病毒感染期间，记忆B细胞的发育和T细胞功能的维持受到损害。IL-21/IL-21R复合物可增强NK细胞的细胞毒性和IFNγ的产生。在人类中，IL-21R基因功能的丧失导致常染色体隐性IL21R免疫缺陷（IL21RID），导致B细胞、T细胞和NK细胞功能障碍，并导致原发性免疫缺陷综合征。
IL-21R is a type I transmembrane protein, belonging to the class I cytokine receptor family. IL-21R contains the WSXWS motif, closely resembling IL-2Rβ and IL-4Rα. Intracellular domain of IL-21R includes the Box 1 and Box 2 elements, which are similar to the IL-9R intracellular region. IL-21R is expressed on B cells and at various levels on NK and T cells, but it is up-regulated after activation. Some studies indicated high levels of IL-21R in some leukemia and lymphoma. IL-21R expression is also elevated by CD40 triggering. IL-21R forms a heterodimer with common γ subunit (CD132), which is shared by the receptors for other interleukins, including IL-2, IL-4, IL-7, IL-9 and IL-15. Upon IL-21 ligation, IL-21R associates with JAK1 and induces phosphorylation cascade and STAT1, 3, 5 transduction pathways. Several evidences shown that IL-21/IL-21R signaling is essential for proliferation and differentiation of B, NK and T cells. In normal B cells, IL-21 mediates cell proliferation, growth arrest, and terminal differentiation, or apoptosis, depending on their activation status. In addition, mice lacking IL-21R have increased production of serum IgE but decreased IgG1. After immunization, antigen-specific IgG production was impaired, indicating IL-21/IL-21R is critical for regulating immunoglobulin production. Though B cell development and acute CD8+ T cell response are normal in IL-21R-deficient mice, development of memory B cells and maintenance of T cell function during chronic viral infection are impaired. IL-21/IL-21R complex can enhance NK cytotoxic activity and IFNγ production. In human, the loss of function in IL-21R gene leads to autosomal recessive IL21R immunodeficiency (IL21RID), resulting in dysfunction of B cells, T cells and NK cells, and a primary immunodeficiency syndrome.

Product Details
Source
Human IL-21R, amino acid (Cys20-Pro236) (Accession: # Q9HBE5.1) with a C-terminal 6His tag, was expressed in CHO cells.

Molecular Mass
The 223 amino acid recombinant protein has a predicted molecular mass of approximately 25.83 kD. The DTT-reduced and non-reduced protein migrates at approximately 50 kD by SDS-PAGE. The predicted N-terminal amino acid is Cys.
Purity  > 95%, as determined by Coomassie stained SDS-PAGE.
Formulation  0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.

Concentration   10-25 µg sizes are bottled at 200 µg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity
The ED50 = 0.02 - 0.10 µg/mL as determined by its ability to inhibit human IL-21-induced IFNγ production on NK92 cells (in the presence of 10 ng/ml of human IL-21, Cat. No. 571202). Human IFN-γ ELISA MAX™ Deluxe Kit (Cat. No. 430104) was used to measure IFNγ production.
Application   Bioassay

Antigen Details
Structure   Cytokine receptor
Distribution
Resting and activated B cells, T cells, NK cells and dendritic cells, activated macrophages, leukemia, lymphoma.

Function
IL-21/IL-21R induces T and B cell proliferation, B cell Ig class switching to IgG production, decreases dendritic cell function, enhances differentiation of effector and central memory T cells, and regulates T cell and hematopoietic progenitor cell homeostasis.

Interaction   JAK
Ligand/Receptor   IL-21
Biology Area   Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, Soluble Receptors

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