BioLegend 573604 Recombinant Mouse CXCL10 (IP-10) (carrier-free)

库存 ：99

英文名 ：BioLegend 573604 Recombinant Mouse CXCL10 (IP-10) (carrier-free)

供应商 ：北京云肽生物科技有限公司

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BioLegend 573604 Recombinant Mouse CXCL10 (IP-10) (carrier-free)
产品简介：
产品品牌：BioLegend
产品货号：573604
产品名称：BioLegend 573604 Recombinant Mouse CXCL10 (IP-10) (carrier-free)
产品规格：25 µg

产品说明：
Regulatory Status   RUO
Other Names
Interferon gamma-inducible protein 10 (IP-10), Interferon gamma induced factor, gIP-10, IFI10, INP10, cytokine responsive gene-2 (CRG2)

描述
CXCL10是一种在结构和功能上与CXCL9和CXCL11相关的ELR阴性趋化因子。CXCL10、CXCL9和CXCL11由单核细胞、巨噬细胞、成纤维细胞和上皮细胞在促炎细胞因子，特别是IFNγ刺激下产生和分泌。CXCL10通过与其受体CXCR3的结合来化学吸引CD4、CD8、NK和NKT细胞，CXCR3与CXCL9和CXCL11共享。此外，CXCL10在体内抑制肿瘤中的新生血管形成和伤口愈合。此外，CXCL10在体外对内皮细胞具有抗增殖作用，在体内具有血管抑制和抗肿瘤作用。已经提出CXCL10在内皮细胞中的抗增殖作用是不依赖于CXCR3的，并且它是通过GAG相互作用介导的。CXCL10还对大肠杆菌和单核细胞增多性李斯特菌以及炭疽杆菌的孢子和芽孢杆菌都具有抗菌活性。CXCL10的表达在许多炎症性疾病中强烈上调，包括关节炎、I型糖尿病、实验性自身免疫性脑脊髓炎、动脉粥样硬化、同种异体移植物排斥反应等。
CXCL10 is an ELR-negative chemokine structurally and functionally related to CXCL9 and CXCL11.  CXCL10, CXCL9, and CXCL11 are produced and secreted by monocytes, macrophages, fibroblasts, and epithelial cells upon stimulation with proinflammatory cytokines, especially IFNγ.  CXCL10 chemoattracts CD4, CD8, and NK and NKT cells through the binding to its receptor CXCR3, which is shared with CXCL9 and CXCL11. In addition, CXCL10 inhibits neovascularization in tumors and in wound healing in vivo. Also, CXCL10 has anti-proliferative effects on endothelial cells in vitro, and angiostatic and antitumor effects in vivo. It has been suggested that the anti-proliferative effect of CXCL10 in endothelial cells is CXCR3-independent and that it is mediated through GAG interaction. CXCL10 also possesses antimicrobial activity against E. coli and L. monocytogenes, and both the spore and bacillus forms of B. anthracis. CXCL10 expression is strongly upregulated in many inflammatory diseases, including arthritis, type I diabetes, experimental autoimmune encepahlomyelitis, atherosclerosis, allograft rejection, and others.

Product Details
Source
Mouse CXCL10, amino acids Ile22-Pro98 (Accession# NM_021274) was expressed in E. coli.

Molecular Mass
The 78 amino acid recombinant protein has a predicted molecular mass of approximately 8.8 kD. The DTT-reduced and non-reduced protein migrate at approximately 10 kD by SDS-PAGE. The N-terminal amino acid is Met.

Purity   >98%, as determined by Coomassie stained SDS-PAGE.
Formulation   0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.

Concentration   10 and 25 µg sizes are bottled at 200 µg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity
Recombinant Mouse CXCL10 (IP-10) induces chemotaxis of mouse Baf3-mCXCR3 transfectant cells in a dose-dependent manner. The ED50 for this effect is < 6 ng /mL.
Application  Bioassay

Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details
Structure   Chemokine
Distribution
Interferon gamma-stimulated keratinocytes, monocytes, macrophages, fibroblasts, endothelial, and T cells.

Function
CXCL10 chemoattracts CD4, Th1, CD8, and NK and NKT cells. CXCL10 is induced by inflammatory cytokines. NH2-terminal cleavage of CXCL10 by DPP-IV/CD26 impairs its chemoattracting capacity and CXCR3 signaling ability.

Interaction
Activated T cells, Th1 cells, regulatory T cells, NK cells, NKT cells, endothelial cells, and fibroblasts.
Ligand/Receptor   CXCR3
Biology AreaCell   Biology, Signal Transduction
Molecular Family   Cytokines/Chemokines

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