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供应商 ：齐源生物
库存 ：999
靶点 ：CD4
级别 ：抗人 CD4流式抗体荧光标记PE
目录编号 ：抗人 CD4流式抗体荧光标记PE
克隆性 ：单克隆
抗原来源 ：抗人 CD4流式抗体荧光标记PE
保质期 ：二年
抗体英文名 ：anti-human CD4 Antibody PE
抗体名 ：CD4
标记物 ：PE
宿主 ：小鼠
适应物种 ：人
免疫原 ：抗人 CD4流式抗体荧光标记PE
亚型 ：抗人 CD4流式抗体荧光标记PE
形态 ：溶液
应用范围 ：FC
保存条件 ：2-8℃
浓度 ：0.2 mg/mL
规格 ：25 tests/100 tests/500 tests/100 µg
抗人 CD4流式抗体荧光标记PE、anti-human CD4 Antibody PE详细如下：Clone：RPA-T4 (See other available formats)
Regulatory Status：RUO
Workshop：IV T114
Other Names：T4
Isotype：Mouse IgG1, κ
Ave. Rating：Submit a Review
Product Citations：39 publications
CD4，也称为 T4，是一种 55 kD 单链 I 型跨膜糖蛋白，在大多数胸腺细胞、T 细胞亚群和单核细胞/巨噬细胞上表达。CD4 是 Ig 超家族的成员，识别与 MHC II 类分子相关的抗原，并参与细胞间相互作用、胸腺分化和信号转导。CD4 是 HIV 的主要受体，与 HIV gp120 结合。CD4 还被证明可以与 IL-16 相互作用。
产品详细：
Verified ReactivityHumanReported ReactivityChimpanzeeAntibody TypeMonoclonalHost SpeciesMouseFormulationµg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).PreparationThe antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.Concentrationµg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)Storage & HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.Application
FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation LaserBlue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of a、cetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
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