供应商 :联科生物
库存 :99
样本 :血清,血浆,细胞培养上清及其他生物学样本
标记物 :HRP
适应物种 :Rat
应用 :ELISA
检测方法 :双抗夹心法
检测限 :3.13 pg/mL - 200 pg/mL
规格 :48T/96T
描述
商品名 |
Rat IL-4 High Sensitivity ELISA Kit (大鼠白细胞介素4高敏 ELISA试剂盒) |
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:5.0% - 6.8%;板间变异系数:2.7% - 7.5% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
0.20 pg/mL |
检测范围 |
3.13 pg/mL - 200 pg/mL |
回收率 |
81% - 101% |
平均回收率 |
0.9 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
- 包被抗IL-4单克隆抗体的96孔聚苯乙烯酶标板
- 大鼠IL-4冻干标准品
- IL-4检测抗体
- 标准品稀释液
- 检测缓冲液(10×)
- 底物(TMB)
- 终止液
- 洗液(20×)
- 封板膜
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-4与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-4的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
标准曲线
分子信息
概述大鼠 IL4, IL-4, BSF1, BCGF1 靶点信息
IL4 分子靶点信息概述
- 分子名:IL4, interleukin 4
- 基因家族:Interleukins
- 别名:BSF1; IL-4; BCGF1; BCGF-1; MGC79402
- 全称:B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1
IL4 分子靶点综述
IL-4是II型辅助T细胞(Th2细胞)分泌的细胞因子。IL-4是一种多效性细胞因子,在调节T、B淋巴细胞和其他类型细胞的增殖、分化、凋亡,促进以Th2细胞为特征的免疫应答过程中发挥重要作用。IL-4多重生物效应依赖于细胞类型和分化状态,研究表明其在介导过敏性疾病、自身免疫性疾病、感染性疾病、肿瘤等疾病的免疫反应中有多重作用,对肿瘤、自身免疫性疾病和感染性疾病等有治疗作用,并且IL-4对疫苗免疫应答具有调节作用。
大鼠 Rat Il4 分子靶点信息
- 分子名:Il4, interleukin 4
- 别称:
- B-cell growth factor 1
- B-cell IGG differentiation factor
- B-cell stimulatory factor 1
- BSF-1
- IL-4
- Il4e12
- interleukin-4
- lymphocyte stimulatory factor 1
- 基因序列:NCBI_Gene: 287287
- 蛋白序列:
- UniprotKB: A0A0G2JVY1
- UniprotKB: P20096
大鼠 Rat Il4 靶点分子功能(预测)
Predicted to enable cytokine activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of leukocyte migration; and regulation of defense response. Located in extracellular space. Used to study anti-basement membrane glomerulonephritis; autoimmune thyroiditis; and pelvic inflammatory disease. Biomarker of several diseases, including cerebrovascular disease (multiple); interstitial lung disease (multiple); irritant dermatitis; primary biliary cholangitis; and rhinitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).
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